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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 490 - 495 (6page)
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Transcription factor AP-2α involves in the process of mammalian embryonic development and tumorigenesis. Many studies have shown that AP-2α functions in association with other interacting proteins. In a two-hybrid screening, the regulatory subunit β of protein casein kinase 2 (CK2β) was identified as an interacting protein of AP-2α; we confirmed this interaction using in-vitro GST pull-down and in-vivo co-immunoprecipitation assays; in an endogenous co-immunoprecipitation experiment,we further found the catalytic subunit α of protein casein kinase 2 (CK2α) also exists in the complex. Phosphorylation analysis revealed that AP-2α was phosphorylated by CK2 kinase majorly at the site of Ser429, and such phosphorylation could be blocked by CK2 specific inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) in a dose-dependent manner.
Luciferase assays demonstrated that both CK2α and CK2β enhanced the transcription activity of AP-2α; moreover, CK2β increased the stability of AP-2α. Our data suggest a novel cellular function of CK-2 as a transcriptional co-activator of AP-2α.
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참고문헌 (23)
참고문헌 신청
Barz, T / 2003 / Genome-wide expression screens indicate a global role for protein kinase CK2 in chromatin remodeling / J. Cell Sci 116 : 1563 ~ 1577
Bosher, J. M / 1996 / A family of AP-2 proteins regulates c-erbB-2 expression in mammary carcinoma / Oncogene 13 : 1701 ~ 1707
Campillos, M / 2003 / Transcriptional activation by AP-2alpha is modulated by the oncogene DEK / Nucleic Acids Res 31 : 1571 ~ 1575
Canton, D. A / 2001 / Assembly of protein kinase CK2: investigation of complex formation between catalytic and regulatory subunits using a zinc-finger-deficient mutant of CK
Cheng, C / 2002 / Cloning and characterization of a novel human transcription factor AP-2 beta like gene (TFAP2BL1) / Int. J. Biochem. Cell Biol 34 : 78 ~ 86
Bosher, J. M / 1996 / A family of AP-2 proteins regulates c-erbB-2 expression in mammary carcinoma / Oncogene 13 : 1701 ~ 1707
Campillos, M / 2003 / Transcriptional activation by AP-2alpha is modulated by the oncogene DEK / Nucleic Acids Res 31 : 1571 ~ 1575
Canton, D. A / 2001 / Assembly of protein kinase CK2: investigation of complex formation between catalytic and regulatory subunits using a zinc-finger-deficient mutant of CK
Cheng, C / 2002 / Cloning and characterization of a novel human transcription factor AP-2 beta like gene (TFAP2BL1) / Int. J. Biochem. Cell Biol 34 : 78 ~ 86
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