지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 182 - 188 (7page)
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초록· 키워드
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Iris dichotoma Pall. is an important endangered plant belonging to the family Iridaceae. A method was developed for the rapid micropropagation of I. dichotoma through plant regeneration from leaf, rhizome, and root explant-derived calli. Leaf, rhizome, and root segments were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D; 0-3.0 mg･L-1) for callus induction. Callus production was highest at 1.0 mg･L-1 2,4-D, where 73.8% and 45.5% of cultured rhizome and root cuttings, respectively, produced calli. The viable calli were maintained at an induced concentration of 2,4-D (3.0 mg･L-1). They were then transferred to MS medium supplemented with various concentrations of 2,4-D (0-3.0 mg･L-1) in combination with 6-benzyladenine (BA: 0, 1.0 and 3.0 mg･L-1) for adventitious shoot regeneration. The addition of a low concentration of 2,4-D into BA-containing medium significantly increased the frequency of shoot regeneration in leaf, rhizome, and root-derived calli. The highest number of adventitious shoots (26.4 per callus) formed at 0.5 mg･L-1 2,4-D and 1.0 mg/l BA. For rooting of the shoots, half- strength MS medium supplemented with different concentrations of indole 3-butyric acid (IBA) 0-3.0 mg･L-1 was tested. The optimal results were observed using half-strength MS medium supplemented with 1.0 mg･L-1 IBA, on which 98% of the regenerated shoots developed roots with an average of 3.5 roots per shoot within 45 days. The plantlets raised in vitro were acclimatized and transferred to soil with 95% success. This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.
목차
등록된 정보가 없습니다.
참고문헌 (38)
참고문헌 신청
Schulze W / 1988 / Wild-Iris für den Garten / Fortschritt Erfurt
Sears RG / 1982 / Tissue culture variability in wheat: callus induction and plant regeneration / Crop Science 22:546~550
Shibli RA / 2000 / Somatic embryogenesis in the endemic black Iris. Plant Cell Tiss Org Cult / Plant Cell Tiss Org Cult 61:15~21
Shimizu K / 1997 / Plant regeneration from suspension culture of Iris germanica / Plant Cell Tissue and Organ Culture 50:27~31
Shimizu K / 1996 / Plant regeneration from protoplasts of Iris germanica L / Euphytica 89:223~227
Sears RG / 1982 / Tissue culture variability in wheat: callus induction and plant regeneration / Crop Science 22:546~550
Shibli RA / 2000 / Somatic embryogenesis in the endemic black Iris. Plant Cell Tiss Org Cult / Plant Cell Tiss Org Cult 61:15~21
Shimizu K / 1997 / Plant regeneration from suspension culture of Iris germanica / Plant Cell Tissue and Organ Culture 50:27~31
Shimizu K / 1996 / Plant regeneration from protoplasts of Iris germanica L / Euphytica 89:223~227
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