지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 37 - 42 (6page)
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초록· 키워드
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Rice is one of the most important cereal crops as a model plant for functional genomics of monocotyledons and usually transformed using Agrobacterium tumefaciens.
However, the transformation’s process using previous method is still time consuming and uneconomical, low efficiency. In this study, we established a new method by modifying the general Agrobacterium protocol especially in the infection and co-cultivation, Agrobacterium elimination,infected calli’s selection steps using liquid media. We directly inoculated Agrobacterium containing a ZjLsL gene under the control of constitutive promoter into the 1- to 3-week-old rice calli derived from mature seeds. After 3days of co-cultivation, the infected calli were transferred onto liquid media of Agrobacterium elimination and calli’s selection for 3 days. The calli were transferred to calli’s growth solid media for 14 days and then the calli transferred to shoot induction and root induction media. Putative transformants were initially selected on the medium containing phosphinothricin, and the PAT protein verified by PAT strip test. This method in this study would lead to reduction of substantial labor and time to generate transgenic plants.
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등록된 정보가 없습니다.
참고문헌 (12)
참고문헌 신청
Christou P / 1991 / Production of transgenic Rice (Oryza Sativa L.) plants from agronomically important indica and japonica varieties via electric discharge particle accelera
Chu CC / 1975 / Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen source / Sci Sin 18 : 659 ~ 668
Datta SK / 1990 / Genetically engineered fertile Indica rice recovered from protoplasts / Bio/Technology 8 : 736 ~ 740
Hiei Y / 1994 / Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA / Plant J 6 : 271 ~ 282
Hirochika H / 1996 / Retrotransposons of rice involved in mutations induced by tissue culture / Proc Nat Acad Sci USA 92 : 8149 ~ 8153
Chu CC / 1975 / Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen source / Sci Sin 18 : 659 ~ 668
Datta SK / 1990 / Genetically engineered fertile Indica rice recovered from protoplasts / Bio/Technology 8 : 736 ~ 740
Hiei Y / 1994 / Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA / Plant J 6 : 271 ~ 282
Hirochika H / 1996 / Retrotransposons of rice involved in mutations induced by tissue culture / Proc Nat Acad Sci USA 92 : 8149 ~ 8153
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