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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 154 - 160 (7page)
이용수
초록· 키워드
오류제보하기
The plant breeding technology was developed with genetic engineering. Many researchers and breeders have turned from traditional breeding to molecular breeding.
Genetically modified organisms (GMO) were developed via molecular breeding technology. Currently, molecular breeding technologies facilitate efficient plant breeding without introducing foreign genes, in virtue by of gene editing technology.
Gene targeting (GT) via homologous recombination (HR) is one of the best gene editing methods available to modify specific DNA sequences in genomes. GT utilizes DNA repair pathways. Thus, DNA repair systems are controlled to enhance HR processing. Engineered sequence specific endonucleases were applied to improve GT efficiency.
Engineered sequence specific endonucleases like the zinc finger nuclease (ZFN), TAL effector nuclease (TALEN), and CRISPR-Cas9 create DNA double-strand breaks (DSB) that can stimulate HR at a target site. RecQl4, Exo1 and Rad51 are effectors that enhance DSB repair via the HR pathway. This review focuses on recent developments in engineered sequence specific endonucleases and ways to improve the efficiency of GT via HR effectors in plants.
목차
등록된 정보가 없습니다.
참고문헌 (64)
참고문헌 신청
Lee KY / 1990 / Homologous recombination in plant cells after Agrobacterium-mediated transformation / Plant Cell Online 2:415~425
Liang Z / 2014 / Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System / J Genet Genomics 41:63~68
Li J-F / 2013 / Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9 / Nat Biotechnol 31:688~691
Li T / 2012 / High-efficiency TALEN-based gene editing produces disease-resistant rice / Nat Biotechnol 30:390~392
Liu Q / 1997 / Design of polydactyl zinc-finger proteins for unique addressing within complex genomes / Proc Natl Acad Sci 94:5525~5530
Liang Z / 2014 / Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System / J Genet Genomics 41:63~68
Li J-F / 2013 / Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9 / Nat Biotechnol 31:688~691
Li T / 2012 / High-efficiency TALEN-based gene editing produces disease-resistant rice / Nat Biotechnol 30:390~392
Liu Q / 1997 / Design of polydactyl zinc-finger proteins for unique addressing within complex genomes / Proc Natl Acad Sci 94:5525~5530
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