지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 116 - 122 (7page)
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초록· 키워드
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Total of fifty accessions of Brassica rapa withvarious morphological characteristics were used for productionof double haploid plants though microspore culture inBrassica rapa. Among them, only 30 accessions inducedembryos from microspores. The highest efficiency of embryoinduction of 1.194 per bud was obtained from IT135449 ofturnip type, while 3 accessions of sarson (winter oil) type didnot generate embryo. The effect of heat shock periods forembryogenesis was also investigated with 4 accessions(IT135449; Turnip type, IT199710; Chinese cabbage type,IT212886; Pak choi type, IT218043; Summer oil type). Thehigh productions of embryos were observed in IT135449,IT199710 and IT212886 when microspores were pre-culturedto 32˚C for 2 days. In IT218043, high embryogenesis wasobserved at the 3 days of heat shock treatment. The optimalcondition of shoot regeneration for IT199710 was observedin MS medium supplemented with NAA 0.5 mg·L-1 andBAP 1 mg·L-1. In contrast, the IT135449 and IT212886 wereobserved high regeneration frequency in MS medium withoutplant growth regulators. All the plantlets regenerated frommicrospore-derived embryos have been successfully transplantedto soil, and bud self-pollinated seeds were produced fromdoubled haploid plants. This indicated that double-haploidgenotype was likely generated naturally during embryogenesisprocess.
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참고문헌 (27)
참고문헌 신청
Wei Z / 2008 / The culture of isolated microspores of ornamental kale (Brassica oleracea var. acephala) and the importance of genotype to embyo regeneration / Scientia Horticul 117:69~72
Zhao J / 2010 / Design of a Brassica rapa core collection for association mapping studies / Genome 53:884~898
Zhao J / 2005 / Genetic relationships within Brassica rapa as inferred from AFLP fingerprints / Theor Appl Genet 110:1301~13041
Zou Z / 2013 / QTL analysis using SNP markers developed by next-generation sequencing for identification of candidate genes controlling 4-methylthio-3-butenyl glucosinolate contents in roots of radish, Raphanus sativus L / PLOS 8:e53541
Baillie AMR / 1992 / In vitro culture of isolated microspores and regeneration of plants in Brassica campestris / Plant Cell Rep 11 : 234 ~ 237
Zhao J / 2010 / Design of a Brassica rapa core collection for association mapping studies / Genome 53:884~898
Zhao J / 2005 / Genetic relationships within Brassica rapa as inferred from AFLP fingerprints / Theor Appl Genet 110:1301~13041
Zou Z / 2013 / QTL analysis using SNP markers developed by next-generation sequencing for identification of candidate genes controlling 4-methylthio-3-butenyl glucosinolate contents in roots of radish, Raphanus sativus L / PLOS 8:e53541
Baillie AMR / 1992 / In vitro culture of isolated microspores and regeneration of plants in Brassica campestris / Plant Cell Rep 11 : 234 ~ 237
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