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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 125 - 131 (7page)
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초록· 키워드
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Pueraria lobata is a perennial legume plant that produces a variety of isoflavones, such as puerarin, daidzin and daidzein. These are metabolized to equol via dihydrodaidzein and tetrahydrodaidzein by the bacterial fermentation of natural isoflavone sources in the human intestines. In this study, we described the growth and accumulation of isoflavone in the hairy root of the Korean wild arrowroot according to the culture period, as well as dihydrodaidzein biosynthesis in hairy root extracts fermented with Pediococcus pentosaceus. Daily proliferation was best in DY1 cultured for 1 week. DY1 showed significant differences in daily production of puerarin and daidzin+daidzein, as compared to DJ7; furthermore, both were best in DY1 cultured for 1 week. The hairy root extract was fermented successfully with P. pentosaceus with confirmed production of dihydrodaidzein, an equol precursor formed by biotransformation. The results indicated that the growth of hairy roots and isoflavone accumulation in the hairy roots is best 1 week after culture. These results are expected to contribute to the mass production of hairy root and isoflavones as equol precursors from the Korean wild arrowroot and provide a basis for equol production by biotransformation in vitro.
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참고문헌 (37)
참고문헌 신청
Arai Y / 2000 / Comparison of isoflavones among dietary intake, plasma concentration and urinary excretion for accurate estimation of phytoestrogen intake / J Epidemiol 10:127~135
Barnes S / 1998 / Evolution of the health benefits of soy isoflavones / Proc Soc Exp Biol Med 217:386~392
Boonsnongcheep P / 2010 / Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures / Plant Cell Tiss Org 101:119~126
Bourgaud F / 1999 / Production of flavonoids by Psoralea hairy root cultures / Plant Cell Tiss Org 56:97~104
Cassidy A / 1996 / Physiological effects of phyto-oestrogens in relation to cancer and other human health risks / Proc Nutr Soc 55:399~417
Barnes S / 1998 / Evolution of the health benefits of soy isoflavones / Proc Soc Exp Biol Med 217:386~392
Boonsnongcheep P / 2010 / Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures / Plant Cell Tiss Org 101:119~126
Bourgaud F / 1999 / Production of flavonoids by Psoralea hairy root cultures / Plant Cell Tiss Org 56:97~104
Cassidy A / 1996 / Physiological effects of phyto-oestrogens in relation to cancer and other human health risks / Proc Nutr Soc 55:399~417
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