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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 110 - 118 (9page)
이용수
초록· 키워드
오류제보하기
We compared germination efficiency for somatic embryos (SE) of Liriodendron tulipifera using semi-solid (SS), temporary immersion bioreactors (TIB), and continuous immersion bioreactors (CIB) to produce vigorous plants. The bioreactors were designed to be immersed in liquid media with plantlets with an adjustable immersion time. TIB and CIB improved germination rates up to 80.86% and 95.21%, respectively, however, CIB produced more hyperhydric plantlets than TIB. The height of plantlets in TIB was significantly higher than for those in CIB. Fresh weights of plantlets grown in CIB of were significantly lower than for those grown in TIB. The lowest chlorophyll concentration was found in in vitro plantlets from CIB. We examined abnormally developed leaves, stems, and apical zones of in vitro plantlets that were produced in CIB. Among the three types, SS showed the highest stomatal density and the shortest stomatal length in in vitro plantlets. After acclimatization, plants from CIB exhibited the lowest values in biomass, such as height, root collar diameter, leaf fresh weight, leaf length, leaf width, petiole length, petiole diameter, and leaf area. Photosynthesis and transpiration rates of ex vitro plants were not significantly different among the three culture types, but stomatal conductance was higher in TIB than in the SS and CIB. Therefore, the results suggest that TIB is the preferable bioreactor to improve in vitro plantlet regeneration of L. tulipifera. TIB-originated plants showed higher growth rate than SS and CIB after transferring to soil.
목차
등록된 정보가 없습니다.
참고문헌 (52)
참고문헌 신청
Abdelmalek EM / 1999 / Effects of sealed and vented gaseous microenvironments on the maturation of somatic embryos of black spruce with a special emphasis on ethylene / Plant Cell Tiss. Org. Cult 56:201~209
Akdemir H / 2014 / Micropropagation of the pistachio and its rootstocks by temporary immersion system / Plant Cell Tiss. Org. Cult 117:65~76
Albarran J / 2005 / Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffee arabica) somatic embryos / Plant Cell Tiss. Org. Cult 81:27~36
Alister B / 2005 / Liquid Culture Systems for in vitro plant propagation :421~442
안찬훈 / 2010 / 생물반응기 내 침지시간에 따른 백합나무 체세포배 발아 및 생화학적 변화 / 한국임학회지 99(3):423~431
Akdemir H / 2014 / Micropropagation of the pistachio and its rootstocks by temporary immersion system / Plant Cell Tiss. Org. Cult 117:65~76
Albarran J / 2005 / Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffee arabica) somatic embryos / Plant Cell Tiss. Org. Cult 81:27~36
Alister B / 2005 / Liquid Culture Systems for in vitro plant propagation :421~442
안찬훈 / 2010 / 생물반응기 내 침지시간에 따른 백합나무 체세포배 발아 및 생화학적 변화 / 한국임학회지 99(3):423~431
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