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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 66 - 75 (10page)
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Agrobacterium-mediated gene transfer has recently been developed to improve rice transformation. In this study, 3 different transformation methods were tested including soaking, co-cultivation, and vacuum infiltration. Agrobacterium tumefaciens GV3101 harboring the binary vector pGreen:: LeGSNOR was used in this experiment. This study aimed to identify the most appropriate method for transferring LeGSNOR into rice. Vacuum infiltration of the embryonic calli for 5 min in Ilpum resulted in high transformation efficiency based on confirmation by PCR, RT-PCR, and qRT-PCR analyses. In conclusion, we described the development of an efficient transformation protocol for the stable integration of foreign genes into rice; furthermore, the study results confirmed that PCR is suitable for efficient detection of the integrated gene. The vacuum infiltration system is a potentially useful tool for future studies focusing on transferring important genes into rice seed calli, and may help reduce time and effort.
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참고문헌 (38)
참고문헌 신청
Aldemita RR / 1996 / Agrobacterium tumefaciensmediated transformation of japonica and indica rice varieties / Planta 199:612~617
Balaji V / 2003 / Agrobacteriummediated transformation efficiency in blackgram and rice enhanced by multiple copies of pTiBo542 virB and virG / Indian J Biotechnol 2:138~146
Bechtold N / 1993 / In-planta Agrobacteriummediated gene transfer by infiltration of adult Arabidopsis thaliana plants / C R Acad Sci Paris Life Sci 316:1194~1199
Bechtold N / 1998 / In-planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration / Methods Mol Biol 82:259~326
Chen EH / 2004 / Factors affecting Agrobacterium-mediated transformation efficiency in rice / Rice Science 11:181~185
Balaji V / 2003 / Agrobacteriummediated transformation efficiency in blackgram and rice enhanced by multiple copies of pTiBo542 virB and virG / Indian J Biotechnol 2:138~146
Bechtold N / 1993 / In-planta Agrobacteriummediated gene transfer by infiltration of adult Arabidopsis thaliana plants / C R Acad Sci Paris Life Sci 316:1194~1199
Bechtold N / 1998 / In-planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration / Methods Mol Biol 82:259~326
Chen EH / 2004 / Factors affecting Agrobacterium-mediated transformation efficiency in rice / Rice Science 11:181~185
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