지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 135 - 140 (6page)
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초록· 키워드
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A less simple approach developed for generation of marker-free transgenic plants is to select transformants without the use of selective marker genes. Some results about development of marker-free transgenic plants were obtained using a non-selective approach in several crops such as rice, potato and tobacco. However, the study did not provide evidence on detailed characterization of introduced gene on genome, a critical step for confirming the stable integration and transmission of a foreign gene. In this study, we evaluated structure and integration sites of transgene (mCry1Ac) in the transgenic Bt rice plants which were made via conventional Agrobacterium-mediated transformation by non-selective method. Structure and integration sites of transgene in these transgenic plants had similar fashion as those recovered under selection.
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등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Bhatnagar M / 2010 / An efficient method for the production of marker-free transgenic plants of peanut (Arachis hypogaea L.) / Plant Cell Rep 29 : 495 ~ 502
Bugert P / 2001 / Improved PCR-walking for large-scale isolation of plant T-DNA borders / BioTechniques 30 : 496 ~ 504
De Buck S / 1999 / The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration / Plant J 20 : 295
De Vetten N / 2003 / A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop / Nat Biotechnol 21 : 439 ~ 442
Doshi KM / 2007 / Anthocyanin expression in marker free transgenic wheat and triticale embryos / In Vitro Cell Dev Biol-Plant 43 : 429 ~ 435
Bugert P / 2001 / Improved PCR-walking for large-scale isolation of plant T-DNA borders / BioTechniques 30 : 496 ~ 504
De Buck S / 1999 / The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration / Plant J 20 : 295
De Vetten N / 2003 / A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop / Nat Biotechnol 21 : 439 ~ 442
Doshi KM / 2007 / Anthocyanin expression in marker free transgenic wheat and triticale embryos / In Vitro Cell Dev Biol-Plant 43 : 429 ~ 435
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