지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 223 - 228 (6page)
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초록· 키워드
오류제보하기
To establish an efficient proliferation and regenerationof PLBs (protocorm-like bodies) of Phalaenopsisplants, a variety of propagation medium types, variousconcentraions of sucrose as well as liquid and solid type weretested in this study. Further, activated charcoal, citric acidand ascorbic acid were compared whether these agents aresuppose to reduce the browning in culture process usingPLBs of Phalanopsis plants. With regard to the properpropagation medium, VW medium showed 1.3 ~ 2 timeshighr than those of other medium in an index of increasingfor fresh weight and 50% higher than those of other mediumin the frequency of shoot regeneration. However, regardingliquid and solid types of culture, there were no significantdifferences in the proliferation of PLBs and regeneration ofshoots from PLBs. In the experiment for a variety of sucroseconcentrations (0 ~ 50 g/l), 10 g of sucrose showed 30 ~ 50%higher than other concentrations in increasing index and10 ~ 50% higher in the regeneration of shoots from PLBs.
Regarding the reduction of browning in tissue culture viaPLBs of Phalaenopsis plants, 1 g of activated charcoal showedonly 1.5% browning of PLBs cultured. Whereas, other treatmentsincluding citric acid and ascorbic acid showed 6 ~ 16% ofbrowning of PLBs. Therefore, activated charcoal was selectedas an efficient anti-browning agents for the culture of PLBsin Phalaenopsis plants. Using above-described results canbe contibuted to the establishment of mass propagation systemusing PLBs of Phalaenopsis plants in the future.
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등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Abdelwahd R / 2008 / Use of an adsorbent and antioxidants to reduce the effects of leached phenolics in in vitro plantlet regeneration of faba bean / Afri J of Biotech 7 (8)
Anthony JM / 2004 / The role of antioxidants for initiation of somatic embryos with Conostephium pendulum (Ericaceae) / Plant Cell Tiss and Org Cult 78 (3) : 247 ~ 252
Carlberg I / 1983 / Improved culture ability of potato protoplasts by use of activated charcoal / Plant Cell Rep 2 : 223 ~ 225
Homma Y / 1985 / New means of Phalaenopsis propagation with internodal zawa sections of flower stalk / J of the Jpn Soc for Hort Sci 54 : 379 ~ 387
Inchihashi S / 1992 / Micropropagation of Phalaenopsis through the culture of lateral buds from young flower stalks / Lindleyana 7 : 208 ~ 215
Anthony JM / 2004 / The role of antioxidants for initiation of somatic embryos with Conostephium pendulum (Ericaceae) / Plant Cell Tiss and Org Cult 78 (3) : 247 ~ 252
Carlberg I / 1983 / Improved culture ability of potato protoplasts by use of activated charcoal / Plant Cell Rep 2 : 223 ~ 225
Homma Y / 1985 / New means of Phalaenopsis propagation with internodal zawa sections of flower stalk / J of the Jpn Soc for Hort Sci 54 : 379 ~ 387
Inchihashi S / 1992 / Micropropagation of Phalaenopsis through the culture of lateral buds from young flower stalks / Lindleyana 7 : 208 ~ 215
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