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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 79 - 83 (5page)
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초록· 키워드
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The purpose of the study was to investigate the antimicrobial activity of the methanol extract of Coptidis rhizome against the type strains of cariogenic bacteria, Streptococcus mutans and Streptococcus sobrinus, and the periodontopathogens, Porphyromonas gingivalis, Prevotella intermedia, Treponema denticola and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of the crude extract and the methanol extract fractions of Coptidis rhizome separated by silica gel chromatography were evaluated by determining the minimal bactericidal concentration (MBC) values, using the microdilution method. The cell viability test of the extracts of Coptidis rhizome on the KB cells was also studied by methyl thiazolyl tetrazolium (MTT) assay. Our results showed that the 11th fraction (F11) of the methanol extract had the greatest antimicrobial activity against the tested bacteria, with no associated cytotoxicity on the KB cells, upto a concentration of 50 μg/ml. These results suggest that the silica gel chromatography fraction F11 of the methanol extract of Coptidis rhizome, could be useful in the development of oral hygiene products as an antimicrobial agent for the prevention of dental caries and periodontal diseases.
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참고문헌 (19)
참고문헌 신청
Anne D. Haffajee / 1994 / Microbial etiological agents of destructive periodontal diseases / Periodontology 2000 5 (1) : 78 ~ 111
Chae SH / 1999 / Growth-inhibiting effects of Coptis japonica root-derived isoquinoline alkaloids on human intestinal bacteria / J Agric Food Chem 47 : 934 ~ 938
Chun Sung Kim / 2013 / Antimicrobial effect of sophoraflavanone G isolated from Sophora flavescens against mutans streptococci / Anaerobe 19 : 17 ~ 21
Darveau RP / 1997 / The microbial challenge in periodontitis / Periodontol 2000 14 : 12 ~ 32
Genco R / 2002 / Periodontal disease and cardiovascular disease: epidemiology and possible mechanisms / J Am Dent Assoc 133 (Suppl) : 14S ~ 22S
Chae SH / 1999 / Growth-inhibiting effects of Coptis japonica root-derived isoquinoline alkaloids on human intestinal bacteria / J Agric Food Chem 47 : 934 ~ 938
Chun Sung Kim / 2013 / Antimicrobial effect of sophoraflavanone G isolated from Sophora flavescens against mutans streptococci / Anaerobe 19 : 17 ~ 21
Darveau RP / 1997 / The microbial challenge in periodontitis / Periodontol 2000 14 : 12 ~ 32
Genco R / 2002 / Periodontal disease and cardiovascular disease: epidemiology and possible mechanisms / J Am Dent Assoc 133 (Suppl) : 14S ~ 22S
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