지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 181 - 188 (8page)
이용수
초록· 키워드
오류제보하기
As the demand for large-scale analysis of gene expression using DNA arrays increases, the importance of the surface characterization of DNA arrays has emerged. We compared the efficiency of molecular biological applications on solid-phases with different surface polarities to identify the most optimal conditions. We employed thiol-gold reactions for DNA immobilization on solid surfaces. The surface polarity was controlled by creating a self-assembled monolayer (SAM)of mercaptohexanol or hepthanethiol, which create hydrophilic or hydrophobic surface properties, respectively. A hydrophilic environment was found to be much more favorable to solid-phase molecular biological manipulations. A SAM of mercaptoethanol had the highest affinity to DNA molecules in our experimetns and it showed greater efficiency in terms of DNA hybridization and polymerization. The optimal DNA concentration for immobilization was found to be 0.5 M. The optimal reaction time for both thiolated DNA and matrix molecules was 10 min and for the polymerase reaction time was 150 min. Under these optimized conditions,molecular biology techniques including DNA hybridization,ligation, polymerization, PCR and multiplex PCR were shown to be feasible in solid-state conditions. We demonstrated from our present analysis the importance of surface polarity in solid-phase molecular biological applications.
A hydrophilic SAM generated a far more favorable environment than hydrophobic SAM for solid‐state molecular techniques. Our findings suggest that the conditions and methods identified here could be used for DNA‐DNA hybridization applications such as DNA chips and for the further development of solid-phase genetic engineering applications that involve DNA-enzyme interactions.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
De Leeuw N / 2011 / SNP array analysis in constitutional and cancer genome diagnostics - copy number variants, genotyping and quality control / Cytogenet Genome Res 135 : 212
Gerhold D / 1999 / DNA chips: promising toys have become powerful tools / Trends Biochem Sci 24 : 168 ~ 173
Gorreta F / 2012 / Genomic profiling: cDNA arrays and oligoarrays / Methods Mol Biol 823 : 89 ~ 105
Gresham D / 2011 / DNA microarray-based mutation discovery and genotyping / Methods Mol Biol 772 : 179 ~ 191
Gronewold T.M. / 2009 / Aptamers and biosensors / Methods Mol Biol 535 : 209 ~ 222
Gerhold D / 1999 / DNA chips: promising toys have become powerful tools / Trends Biochem Sci 24 : 168 ~ 173
Gorreta F / 2012 / Genomic profiling: cDNA arrays and oligoarrays / Methods Mol Biol 823 : 89 ~ 105
Gresham D / 2011 / DNA microarray-based mutation discovery and genotyping / Methods Mol Biol 772 : 179 ~ 191
Gronewold T.M. / 2009 / Aptamers and biosensors / Methods Mol Biol 535 : 209 ~ 222
이 논문의 저자 정보
최근 본 자료
댓글(0)
0