지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한병리학회 Journal of Pathology and Translational Medicine Journal of Pathology and Translational Medicine 제45권 제5호
- 발행연도
- 2011.1
- 수록면
- 469 - 476 (8page)
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초록· 키워드
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Background: Although the importance of aberrant DNA methylation in the development of gastric adenocarcinoma has been described, the mechanism of pathogenesis has not been revealed yet. We quantitatively analyzed methylation of four CpG islands and one repetitive DNA element, according to the histologic features of adenocarcinoma with precursor lesions. Methods: We divided the cases as adenocarcinoma with intestinal type precursors (type A, n=19 cases) and adenocarcinoma with diffuse type precursors (type B, n=19 cases). We micro-dissected tumor cells and matched non-neoplastic gastric mucosa from the hematoxylin and eosin-stained slides. Results: A total of 20 CpG sites of long interspersed nucleotide element-1 (LINE1), RAR-related orphan receptor alpha (RORA), Kruppel-like factor 7 (KLF7), mutL homolog 1 (MLH1), MINT25, and CD133 were analyzed. Methylation was determined by bisulfate-pyro-sequencing, and hypomethylation of LINE1 and CD133 was noted in the tumors, compared to the levels in the non-neoplastic gastric mucosa (p=0.014 and p=0.015, respectively). A statistically different methylation pattern of CpG sites at CD133 and KLF7 was noted only in type B lesions, compared to that in matched non-neoplastic gastric mucosa (p=0.027 and p=0.043, respectively). Conclusions: Given that aberrant methylation occurs in a relatively early phase of carcinogenesis, different patterns of methylation may determine the carcinoma phenotype. However, further large-scale study is required to clarify the significance of this difference.
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참고문헌 (31)
참고문헌 신청
Bieker JJ / 2001 / Krüppel-like factors: three fingers in many pies / J Biol Chem 276:34355~34358
Maekita T / 2006 / High levels of aberrant DNA methylation in Helicobacter pylori-infected gastric mucosae and its possible association with gastric cancer risk / Clin Cancer Res 12(3 Pt 1):989~995
Qian X / 2008 / E-cadherin promoter hypermethylation induced by interleukin-1beta treatment or H. pylori infection in human gastric cancer cell lines / Cancer Lett 263:107~113
/ 200906 / Sensitive and Specific Detection of Early Gastric Cancer with DNA Methylation Analysis of Gastric Washes / Gastroenterology 136 : 2149 ~ 2158
Baba T / 2009 / Epigenetic regulation of CD133 and tumorigenicity of CD133+ ovarian cancer cells / Oncogene 28 : 209 ~ 218
Maekita T / 2006 / High levels of aberrant DNA methylation in Helicobacter pylori-infected gastric mucosae and its possible association with gastric cancer risk / Clin Cancer Res 12(3 Pt 1):989~995
Qian X / 2008 / E-cadherin promoter hypermethylation induced by interleukin-1beta treatment or H. pylori infection in human gastric cancer cell lines / Cancer Lett 263:107~113
/ 200906 / Sensitive and Specific Detection of Early Gastric Cancer with DNA Methylation Analysis of Gastric Washes / Gastroenterology 136 : 2149 ~ 2158
Baba T / 2009 / Epigenetic regulation of CD133 and tumorigenicity of CD133+ ovarian cancer cells / Oncogene 28 : 209 ~ 218
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