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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 161 - 168 (8page)
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Objective To analyze both differentially expressed genes and the Bcl-xL protein expression after acute and chronic treatment with fluoxetine in rat C6 glioma cells.
Methods C6 glioma cells were cultured for 24 h or 72 h after treatment with 10 μM fluoxetine, and gene expression patterns were observed using microarray and qRT-PCR. Then, cells were cultured for 6 h, 24 h, 72 h or 96 h after treatment with 10 μM fluoxetine, and the expression of Bcl-xL protein was measured using western blot.
Results As determined by microarray, treatment with fluoxetine for 24 h up-regulated 33 genes (including Bcl-xL and NCAM140)and down-regulated 7 genes (including cyclin G-associated kinase). Treatment with fluoxetine for 72 h up-regulated 53 genes (including Gsαand Bcl-xL) and down-regulated 77 genes (including Gαi2 and annexin V). Based on the qRT-PCR results, there was an increase in GsαmRNA and a decrease in Gαi2 mRNA at 72 h in fluoxetine-treated cells as compared to control, a result that was consistent with microarray.
We also observed an increase in Bcl-xL mRNA (both at 24 h and at 72 h) in fluoxetine-treated cells as compared to control, demonstrating a tendency to increase gradually. Bcl-xL protein expression increased as the duration of fluoxetine treatment increased.
Conclusion These results suggest that chronic treatment with fluoxetine not only initiates the cAMP pathway through inducing Gsα expression but also induces Bcl-xL expression, thus inhibiting apoptosis.
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참고문헌 (23)
참고문헌 신청
Baik SY / 2005 / Fluoxetine- induced up-regulation of 14-3-3zeta and tryptophan hydroxylase levels in RBL-2H3 cells / Neurosci Lett 374 : 53 ~ 57
Chen J / 1995 / Chronic treatment of C6 glioma cells with antidepressant drugs increases functional coupling between a G protein (Gs) and adenylyl cyclase / J Neurochem 64 :
Chiou SH / 2006 / Fluoxetine up-regulates expression of cellular FLICE-inhibitory protein and inhibits LPS-induced apoptosis in hippocampus-derived neural stem cell / Biochem
Donati RJ / 2001 / Chronic treatment of C6 glioma cells with antidepressant drugs results in a redistribution of Gsalpha / Mol Pharmacol 59 : 1426 ~ 1432
Dowlatshahi D / 1999 / G Protein- coupled cyclic AMP signaling in postmortem brain of subjects with mood disorders: effects of diagnosis, suicide, and treatment at the time
Chen J / 1995 / Chronic treatment of C6 glioma cells with antidepressant drugs increases functional coupling between a G protein (Gs) and adenylyl cyclase / J Neurochem 64 :
Chiou SH / 2006 / Fluoxetine up-regulates expression of cellular FLICE-inhibitory protein and inhibits LPS-induced apoptosis in hippocampus-derived neural stem cell / Biochem
Donati RJ / 2001 / Chronic treatment of C6 glioma cells with antidepressant drugs results in a redistribution of Gsalpha / Mol Pharmacol 59 : 1426 ~ 1432
Dowlatshahi D / 1999 / G Protein- coupled cyclic AMP signaling in postmortem brain of subjects with mood disorders: effects of diagnosis, suicide, and treatment at the time
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