지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2006.1
- 수록면
- 224 - 228 (5page)
이용수
초록· 키워드
오류제보하기
Recent studies have suggested that p53 regulates the G2 checkpoint in the cell cycle and this function is required for the maintenance of genomic integrity. In this study, we addressed a role of p53 in escaping from cell cycle G2 arrest following DNA damage.
Materials and Methods: Cell cycle checkpoint arrest in the human colon cancer cell line HCT116 and its derivatives carry p53 or p21 deletions, were examined by FACS analysis, immunoprecipitation, Western blot and IP-kinase assay.
Results: While the cells with functional p53 were arrested at both the G1 and G2 checkpoints, the p53-deficient cells failed to arrest at G1, but they were arrested at G2. However, the p53-deficient cells failed to sustain G2 checkpoint arrest and they entered mitosis earlier than did the p53-positive cells and so this resulted in extensive cell death. Cdc2 kinase becomes reactivated in p53-deficient cells in association with entry into mitosis, but not in the p53-positive cells. Upon DNA damage, the p21-deficient cells, like the p53-negative cells, not only failed to repress cdk2- dependent NF-Y phosphorylation, but they also failed to repress the expression of such cell cycle G2-regulatory genes as cdc2, cyclin B, RNR-R2 and cdc25C, which have all been previously reported as targets of NF-Y transcription factor.
Conclusion: p53 is essential to prevent immature escaping from cell cycle G2 checkpoint arrest through p21-mediated cdk2 inactivation, and this leads to inhibition of cdk2-dependent NF-Y phosphorylation and NF-Y dependent transcription of the cell cycle G2-rgulatory genes, including cdc2 and cyclin B. (Cancer Res Treat. 2006;38:224-228)
목차
등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
/ 1989;246 / Checkpoints : controls that ensure the order of cell cycle events : 629 ~ 34
/ 1991;253 / p53 mutations in human cancers : 49 ~ 53
/ 1992;89 / Wild-type p53 is a cell cycle checkpoint determinant following irradiation : 7491 ~ 5
/ 1993 / WAF1, a potential mediator of p53 tumor suppression (t) : 817 ~ 25
/ 1995 / p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts 92 : 8493 ~ 8497
/ 1991;253 / p53 mutations in human cancers : 49 ~ 53
/ 1992;89 / Wild-type p53 is a cell cycle checkpoint determinant following irradiation : 7491 ~ 5
/ 1993 / WAF1, a potential mediator of p53 tumor suppression (t) : 817 ~ 25
/ 1995 / p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts 92 : 8493 ~ 8497
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Cadmium Induces Cell Cycle Arrest and Change in Expression of Cell Cycle Related Proteins in Breast Cancer Cell Lines
Toxicological Research
2005 .03
-
The Role of Cell Cycle Regulators in Normal and Malignant Cell Proliferation
대한의생명과학회지
2010 .06
-
Involvement of Cdc25c in Cell Cycle Alteration of a Radioresistant Lung Cancer Cell Line Established with Fractionated Ionizing Radiation
Asian Pacific journal of cancer prevention : APJCP
2013 .01
-
S Phase Cell Cycle Arrest and Apoptosis is Induced by Eugenol in G361 Human Melanoma Cells
International Journal of Oral Biology
2011 .01
-
Comparative Studies to Evaluate Relative in vitro Potency of Luteolin in Inducing Cell Cycle Arrest and Apoptosis in HaCaT and A375 Cells
Asian Pacific journal of cancer prevention : APJCP
2013 .01
-
Effect of benzo(a)pyrene and mitomycine C on HeLa cell division cycle
한국환경성돌연변이·발암원학회지
2001 .09
-
High-Dose $^{111}In$ Induces G1 Cell Cycle Arrest and Cell Death in Rat Bone Marrow Mesenchymal Stem Cells
Nuclear medicine and molecular imaging : NMMI
2012 .01
-
Cancer and Cell Cycle
Annals of Hepato-Biliary-Pancreatic Surgery
1997 .12
-
Panaxadiol Arrests Cell Cycle by Elevating $p21^{WAF1/CIP1}$
대한약학회 학술대회
2003 .01
-
Cell Cycle Regulatory Protein Expression Profiles by Adenovirus p53 Infection in Human Papilloma Virus-associated Cervical Cancer Cells
Cancer Research and Treatment
2006 .01
-
Induction of cell cycle arrest and apoptosis by an indirubin analog, a CDK inhibitor, in human lung cancer cells
대한약학회 학술대회
2003 .01
-
Cell cycle arrest of alveolar epithelial cells is critical in the pathogenesis of idiopathic pulmonary fibrosis
한국실험동물학회 학술발표대회 논문집
2018 .07
-
Differences in Their Proliferation and Differentiation between B-1 and B-2 Cell
Immune Network
2006 .03
-
In Vitro Anti-Neuroblastoma Activity of Thymoquinone Against Neuro-2a Cells via Cell-cycle Arrest
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Amygdalin Modulates Cell Cycle Regulator Genes in Human Chronic Myeloid Leukemia Cells
Molecular & cellular toxicology
2006 .01
-
RNA-sequencing Profiles of Cell Cycle–Related Genes Upregulated during the G2-Phase in Giardia lamblia
Parasites, Hosts and Diseases
2019 .04
-
Panduratin A Inhibits Cell Proliferation by Inducing G0/G1 Phase Cell Cycle Arrest and Induces Apoptosis in Breast Cancer Cells
Biomolecules & Therapeutics(구 응용약물학회지)
2018 .01
-
Radical Intermediate Generation and Cell Cycle Arrest by an Aqueous Extract of Thunbergia Laurifolia Linn. in Human Breast Cancer Cells
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Cell Cycle, Cell Death and Apoptosis
대한핵의학회 연수교육
2002 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0