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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 297 - 306 (10page)
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PurposeInterleukin-17 (IL-17) is a proinflammatory cytokine that plays important roles ininflammation, autoimmunity, and cancer. The purpose of this study was to determineif IL-17 indirectly regulates macrophage differentiation through up-regulation ofcyclooxygenase-2 (COX-2) expression in the cancer cell lines.
Materials and MethodsHuman cervical cancer HeLa, human lung cancer A549, and mouse prostate cancerMyc-CaP/CR cell lines were treated with recombinant IL-17; Western blot analysis,enzyme-linked immunosorbent assay, and quantitative real-time polymerase chainreaction analysis were utilized to examine the cellular responses.
ResultsIL-17 up-regulated expression of COX-2 mRNA and protein in HeLa, A549, and Myc-CaP/CR cell lines. IL-17’s effects were mediated through nuclear factor-κB andERK1/2 signaling pathways as the inhibitors of these pathways could inhibit IL-17-induced COX-2 expression. The conditional medium obtained from the cancer cellscontained prostaglandin E2, the levels of which were increased by IL-17 treatment.
When treated with the conditional medium, particularly with the IL-17-induced conditionalmedium, mouse RAW264.7 macrophages and human THP-1 monocytes expressedhigher levels of IL-10 (a marker of M2 macrophages) than inducible nitricoxide synthase or tumor necrosis factor α (markers of M1 macrophages). In contrast,when RAW264.7 and THP-1 cells were treated directly with IL-17, expression of thesemarker genes was not markedly changed.
ConclusionThe results of this study suggest that IL-17 indirectly promotes M2 macrophagedifferentiation through stimulation of the COX-2/PGE2 pathway in the cancer cells,thus IL-17 plays an indirect role in regulating the tumor immune microenvironment.
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참고문헌 (24)
참고문헌 신청
Chen WC / 2013 / Interleukin-17-producing cell infiltration in the breast cancer tumour microenvironment is a poor prognostic factor / Histopathology 63 : 225 ~ 233
Chen Z / 2013 / The Th17/Treg balance and the expression of related cytokines in Uygur cervical cancer patients / Diagn Pathol 8 : 61 ~
Chung AS / 2013 / An interleukin-17-mediated paracrine network promotes tumor resistance to anti-angiogenic therapy / Nat Med 19 : 1114 ~ 1123
Ellis L / 2012 / Development of a castrate resistant transplant tumor model of prostate cancer / Prostate 72 : 587 ~ 591
Gordon S / 2003 / Alternative activation of macrophages / Nat Rev Immunol 3 : 23 ~ 35
Chen Z / 2013 / The Th17/Treg balance and the expression of related cytokines in Uygur cervical cancer patients / Diagn Pathol 8 : 61 ~
Chung AS / 2013 / An interleukin-17-mediated paracrine network promotes tumor resistance to anti-angiogenic therapy / Nat Med 19 : 1114 ~ 1123
Ellis L / 2012 / Development of a castrate resistant transplant tumor model of prostate cancer / Prostate 72 : 587 ~ 591
Gordon S / 2003 / Alternative activation of macrophages / Nat Rev Immunol 3 : 23 ~ 35
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