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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한약리학회 The Korean Journal of Physiology & Pharmacology The Korean Journal of Physiology & Pharmacology 제15권 제5호
- 발행연도
- 2011.1
- 수록면
- 273 - 277 (5page)
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초록· 키워드
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Nitric oxide (NO) and atrial natriuretic peptide (ANP) may induce vascular relaxation by increasing the production of cyclic guanosine monophosphate (cGMP), an important mediator of vascular tone during sepsis. This study aimed to determine whether regulation of NO and the ANP system is altered in lipopolysaccharide (LPS)-induced kidney injury. LPS (10 mgㆍkg−1) was injected in the tail veins of male Sprague-Dawley rats; 12 hours later, the kidneys were removed. Protein expression of NO synthase (NOS) and neutral endopeptidase (NEP) was determined by semiquantitative immunoblotting. As an index of synthesis of NO, its stable metabolites (nitrite/nitrate, NOx) were measured using colorimetric assays. mRNA expression of the ANP system was determined by real-time polymerase chain reaction. To determine the activity of guanylyl cyclase (GC), the amount of cGMP generated in response to sodium nitroprusside (SNP) and ANP was calculated. Creatinine clearance decreased and fractional excretion of sodium increased in LPS-treated rats compared with the controls. Inducible NOS protein expression increased in LPS-treated rats, while that of endothelial NOS, neuronal NOS, and NEP remained unchanged. Additionally, urinary and plasma NOx levels increased in LPS-treated rats. SNP-stimulated GC activity remained unchanged in the glomerulus and papilla in the LPS-treated rats. mRNA expression of natriuretic peptide receptor (NPR)-C decreased in LPS-treated rats, while that of ANP and NPR-A did not change. ANP-stimulated GC activity reduced in the glomerulus and papilla. In conclusion, enhancement of the NO/cGMP pathway and decrease in ANP clearance were found play a role in the pathogenesis of LPS-induced kidney injury.
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참고문헌 (23)
참고문헌 신청
Aiura K / 1995 / Circulating concentrations and physiologic role of atrial natriuretic peptide during endotoxic shock in the rat / Crit Care Med 23 : 1898 ~ 1906
Bachmann S / 1995 / Topography of nitric oxide synthesis by localizing constitutive NO synthases in mammalian kidney / Am J Physiol 268 : 885 ~ 898
Carnio EC / 2005 / Vasopressin release during endotoxaemic shock in mice lacking inducible nitric oxide synthase / Pflugers Arch 450 : 390 ~ 394
Chinkers M / 1989 / The protein kinase domain of the ANP receptor is required for signaling / Science 245 : 1392 ~ 1394
De Bold AJ / 1981 / A rapid and potent natriuretic response to intravenous injection of atrial myocardial extract in rats / Life Sci 28 : 89 ~ 94
Bachmann S / 1995 / Topography of nitric oxide synthesis by localizing constitutive NO synthases in mammalian kidney / Am J Physiol 268 : 885 ~ 898
Carnio EC / 2005 / Vasopressin release during endotoxaemic shock in mice lacking inducible nitric oxide synthase / Pflugers Arch 450 : 390 ~ 394
Chinkers M / 1989 / The protein kinase domain of the ANP receptor is required for signaling / Science 245 : 1392 ~ 1394
De Bold AJ / 1981 / A rapid and potent natriuretic response to intravenous injection of atrial myocardial extract in rats / Life Sci 28 : 89 ~ 94
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