IL-4 and IL-5 Secretions Predominate in the Airways of Wistar Rats Exposed to Toluene Diisocyanate Vapor

Kouame Kouadio; Kui-Cheng Zheng; Abdoulaye Abba Toure; Mireille Dosso; Hidemi Todoriki

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자료유형: 학술저널

저자정보: Kouame Kouadio Kui-Cheng Zheng Abdoulaye Abba Toure Mireille Dosso Hidemi Todoriki

저널정보: 대한예방의학회 예방의학회지 예방의학회지 제47권 제1호

발행연도: 2014.1

수록면: 57 - 63 (7page)

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Objectives: We established a Wistar rat model of asthma caused by toluene diisocyanate (TDI) exposure, and investigated the relationshipbetween TDI exposure concentrations and respiratory hypersensitivity, airway inflammation, and cytokine secretions in animals,to better understand the mechanism of TDI induced occupational asthma. Methods: Wistar rats were exposed to two different concentrations of TDI vapor four hours a day for five consecutive days. Bronchoalveolarlavage (BAL) was performed, and differential leucocytes from the BAL fluid were analyzed. Lung histopathological examination was carried out to investigate the inflammatory status in the airways. Production of cytokines interleukin (IL)-4 and IL-5 productions in the BAL fluid in vivo was determined with enzyme-linked immunosorbent assay kits. Results: The TDI-exposed rats exhibited greater airway hypersensitivity symptoms than the control rats. The BAL differential cell count and lung histopathological examination demonstrated that inflammation reactions were present in both the central and peripheral airways, characterized with marked infiltration of eosinophils in the TDI-exposed rats. The cytokine assay showed that IL-4 and IL-5 were predominantly produced in the BAL fluid in vivo. Conclusions: These findings imply that TDI exposure concentrations may greatly affect the occurrence and extent of inflammatory events and that Th2 type cytokines may play an important role in the immunopathogenesis of TDI-induced occupational respiratory hypersensitivity.

#Toluene 2 #4-diisocyanate #Wistar rat #Bronchoalveolar lavage #Cytokines #Eosinophils #Airway

참고문헌 (37)

참고문헌 신청

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