지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Objectives: Nail has been a substitute DNA source for genotyping. To investigate the integrity and consistency of nail DNA amplification for biomarker study, nail clippings from 12 subjects were collected at monthly intervals. The possibility of longer amplification and existence of GAPDH RNA/protein, were also investigated with three nail samples.
Methods: Three primer sets were designed for quantitative amplification of nuclear and mitochondrial genes and analysis of their consistency.
The mean threshold cycles in amplification of the target genes were compared to test the consistency of polymerase chain reaction (PCR) performance among individual factors including age groups, sex, family, the nail source, and by the size of the amplification segments.
Results: The amplification of the target genes from nail DNA showed similar integrity and consistency between the nail sources, and among the serial collections. However, nail DNA from those in their forties showed earlier threshold cycles in amplification than those in their teens or seventies. Mitochondrial DNA (mtDNA) showed better DNA integrity and consistency in amplification of all three targets than did nuclear DNA (nucDNA). Over 9 kb of mtDNA was successfully amplified, and nested quantitative PCR showed reliable copy numbers (%) between the two loci. Reverse transcription PCR for mRNA and immunoblotting for GAPDH protein successfully reflected their corresponding amounts. Regarding the existence of RNA and protein in nails, more effective extraction and detection methods need to be set up to validate the feasibility in biomarker study.
Conclusions: Nail DNA might be a feasible intra-individual monitoring biomarker. Considering integrity and consistency in target amplification,mtDNA would be a better target for biomarker research than nucDNA.
목차
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참고문헌 (35)
참고문헌 신청
Tong YK / 2006 / Diagnostic developments involving cell-free (circulating) nucleic acids / Clin Chim Acta 363(1):187~196
Pinzani P / 2010 / Circulating nucleic acids in cancer and pregnancy / Methods 50(4):302~307
Zachariah R / 2009 / Circulating cell-free DNA as a potential biomarker for minimal and mild endometriosis / Reprod Biomed Online 18(3):407~411
Zitt M / 2008 / Circulating cell-free DNA in plasma of locally advanced rectal cancer patients undergoing preoperative chemoradiation: a potential diagnostic tool for therapy monitoring / Dis Markers 25(3):159~165
Lakshmi Priya MD / 2011 / Level of trace elements (copper, zinc, magnesium and selenium) and toxic elements (lead and mercury) in the hair and nail of children with autism / Biol Trace Elem Res 142(2):148~158
Pinzani P / 2010 / Circulating nucleic acids in cancer and pregnancy / Methods 50(4):302~307
Zachariah R / 2009 / Circulating cell-free DNA as a potential biomarker for minimal and mild endometriosis / Reprod Biomed Online 18(3):407~411
Zitt M / 2008 / Circulating cell-free DNA in plasma of locally advanced rectal cancer patients undergoing preoperative chemoradiation: a potential diagnostic tool for therapy monitoring / Dis Markers 25(3):159~165
Lakshmi Priya MD / 2011 / Level of trace elements (copper, zinc, magnesium and selenium) and toxic elements (lead and mercury) in the hair and nail of children with autism / Biol Trace Elem Res 142(2):148~158
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