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In this study, a co-axial flow induced microfluidic chip to fabricate pure collagen type I microfiber via the control of collagen type I and Na-alginate gelation process. The pure collagen type I microfiber was generated by selective degradation of Ca-alginate from ‘Core-Shell’ structured hydrogel microfiber. To make ‘Core-Shell’ structure, collagen type I solution was introduced into core channel and 1.5% Na-alginate solution was injected into side channel in microfluidic chip. To evaluatethe ‘Core-Shell’ structure, the red and green fluorescence substances were mixed into collagen type I and Na-alginate solution, respectively. The fluorescence substances were uniformly loaded into each fiber, and the different fluorescence images were dependent on their location. By immoblizing EpH4-Ras and C6 cells within collagen type I and Na-alginate solution, we sucessfully demonstrated the co-culture of EpH4-Ras and C6 cells with ‘Core-Shell’ like hydrogel microfiber for 5 days. Only to produce pure collagen type I hydrogel fiber, tri-sodium citrate solution was used to dissolve the shell-like Ca-alginate hydrogel fiber from ‘Core-Shell’ structured hydrogel microfiber, which is an excellent advantage when the fiber is employed in three-dimensional scaffold. This novel method could apply variousapplication in tissue engineering and biomedical engineering
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참고문헌 (21)
참고문헌 신청
Q. L. Loh / 2013 / Three-Dimensional Scaffolds for Tissue Engineering Applications : Role of Porosity and Pore Size / Tissue Engineering Part B-Reviews 19:485~502
P. Eiselt / 2000 / Porous carriers for biomedical applications based on alginate hydrogels / Biomaterials 21:1921~1927
E. Carletti / 2011 / Scaffolds for tissue engineering and 3D cell culture / Methods Mol Biol 695:17~39
F. J. O’Brien / 2011 / Biomaterials & scaffolds for tissue engineering / Materials Today 14:88~95
R. Langer / 2000 / Biomaterials in drug delivery and tissue engineering : one laboratory’s experience / Acc Chem Res 33:94~101
P. Eiselt / 2000 / Porous carriers for biomedical applications based on alginate hydrogels / Biomaterials 21:1921~1927
E. Carletti / 2011 / Scaffolds for tissue engineering and 3D cell culture / Methods Mol Biol 695:17~39
F. J. O’Brien / 2011 / Biomaterials & scaffolds for tissue engineering / Materials Today 14:88~95
R. Langer / 2000 / Biomaterials in drug delivery and tissue engineering : one laboratory’s experience / Acc Chem Res 33:94~101
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