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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 202 - 208 (7page)
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초록· 키워드
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Background: Lung cancer is one of the leading causes of cancer-related deaths throughout the world. The gene tumor protein 53 (TP53) is frequently mutated in cases of lung cancer. This study was performed to investigate the frequencies and types of mutations in the TP53 gene in Korean patients with lung cancer.
Methods: We obtained tissue samples from 80 lung cancer patients and synthesized TP53 cDNA by using RNA isolated from these tissues by performing reverse transcriptase polymerase chain reaction. Hybridization and denaturing high-performance liquid chromatography were performed to identify the TP53 gene mutations, and then, the mutations were validated by direct sequencing.
Results: Forty mutations out of the 80 patients (50.0%) were noted in the TP53 gene. The frequencies of TP53 gene mutation for different cancer types, namely, squamous cell carcinoma, adenocarcinoma, and small cell carcinoma were 61.1%, 27.3%, and 26.7%, respectively. The mutation frequencies in the different regions of the gene were 10.0% for exon 4, 35.0% for exon 5, 12.5% for exon 6, 22.5% for exon 7, 17.5% for exon 8,and 2.5% for exon 9. The frequently mutated positions were codon 179 in exon 5, codons 202 and 220 in exon 6, and codons 266 and 273 in exon 8.
Conclusions: Exon 5 was the most frequently mutated region in the TP53 gene. Compared to the patients with the other types of cancers, patients with squamous cell carcinoma showed a higher frequency of TP53 mutation. Codon 179 was the most frequently mutated codon in the TP53 gene.
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참고문헌 (24)
참고문헌 신청
Bennett WP / 1999 / Molecular epidemiology of human cancer risk: gene-environment interactions and p53 mutation spectrum in human lung cancer / J Pathol 187 : 8 ~ 18
Feng Z / 2003 / Chromium(VI) exposure enhances polycyclic aromatic hydrocarbon-DNA binding at the p53 gene in human lung cells / Carcinogenesis 24 : 771 ~ 778
Hainaut P / 2000 / p53 and human cancer: the first ten thou-sand mutations / Adv Cancer Res 77 : 81 ~ 137
Hashimoto T / 2000 / Different subtypes of human lung adenocarcinoma caused by different etiological factors. Evidence from p53 mutational spectra / Am J Pathol 157 : 2133 ~
Health Organization / / International Agency for Research on Cancer. IARC tp53 database, R15
Feng Z / 2003 / Chromium(VI) exposure enhances polycyclic aromatic hydrocarbon-DNA binding at the p53 gene in human lung cells / Carcinogenesis 24 : 771 ~ 778
Hainaut P / 2000 / p53 and human cancer: the first ten thou-sand mutations / Adv Cancer Res 77 : 81 ~ 137
Hashimoto T / 2000 / Different subtypes of human lung adenocarcinoma caused by different etiological factors. Evidence from p53 mutational spectra / Am J Pathol 157 : 2133 ~
Health Organization / / International Agency for Research on Cancer. IARC tp53 database, R15
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