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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 195 - 201 (7page)
이용수
초록· 키워드
오류제보하기
Objectives The use of chitosan nanoparticles (CNPs) in endodontics is of interest due to their antibiofilm properties. This study was to investigate the ability of bioactive CNPs to remove the smear layer and inhibit bacterial recolonization on dentin.
Materials and Methods One hundred bovine dentin sections were divided into five groups (n = 20 per group) according to the treatment. The irrigating solutions used were 2.5% sodium hypochlorite (NaOCl) for 20 min, 17% ethylenediaminetetraacetic acid (EDTA) for 3 min and 1.29 mg/mL CNPs for 3 min. The samples were irrigated with either distilled water (control), NaOCl, NaOCl-EDTA, NaOCl-EDTA-CNPs or NaOCl-CNPs. After the treatment, half of the samples (n = 50) were used to assess the chelating effect of the solutions using portable scanning electronic microscopy, while the other half (n = 50) were infected intra-orally to examine the post-treatment bacterial biofilm forming capacity. The biovolume and cellular viability of the biofilms were analysed under confocal laser scanning microscopy. The Kappa test was performed for examiner calibration, and the non-parametric Kruskal-Wallis and Dunn tests (p < 0.05) were used for comparisons among the groups.
Results The smear layer was significantly reduced in all of the groups except the control and NaOCl groups (p < 0.05). The CNPs-treated samples were able to resist biofilm formation significantly better than other treatment groups (p < 0.05).
Conclusions CNPs could be used as a final irrigant during root canal treatment with the dual benefit of removing the smear layer and inhibiting bacterial recolonization on root dentin.
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등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Calamari SE / 2011 / Antifungal and post-antifungal effects of chlorhexidine, fluconazole, chitosan and its combinations on Candida albicans / Med Oral Patol Oral Cir Bucal
Calt S / 2002 / Time-dependent effects of EDTA on dentin structures / J Endod 28 : 17 ~ 19
Chavez de Paz LE / 2009 / Image analysis software based on color segmentation for characterization of viability and physiological activity of biofilms / Appl Environ Microbi
Costerton JW / 1999 / Bacterial biofilms: a common cause of persistent infections / Science 284 : 1318 ~ 1322
De Moura MR / 2009 / Improved barrier and mechanical properties of novel hydroxypropyl methylcellulose edible films with chitosan/tripolyphosphate nanoparticles / J Food Eng
Calt S / 2002 / Time-dependent effects of EDTA on dentin structures / J Endod 28 : 17 ~ 19
Chavez de Paz LE / 2009 / Image analysis software based on color segmentation for characterization of viability and physiological activity of biofilms / Appl Environ Microbi
Costerton JW / 1999 / Bacterial biofilms: a common cause of persistent infections / Science 284 : 1318 ~ 1322
De Moura MR / 2009 / Improved barrier and mechanical properties of novel hydroxypropyl methylcellulose edible films with chitosan/tripolyphosphate nanoparticles / J Food Eng
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