지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치주과학회 Journal of Periodontal & Implant Science Journal of Periodontal & Implant Science 제41권 제3호
- 발행연도
- 2011.1
- 수록면
- 157 - 163 (7page)
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초록· 키워드
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Purpose: Curcumin is known to exert numerous biological effects including anti-inflammatory activity. In this study, we investigated the effects of curcumin on the production of interleukin-6 (IL-6) by murine macrophage-like RAW 264.7 cells stimulated with lipopolysaccharide (LPS) from Prevotella intermedia, a major cause of inflammatory periodontal disease, and sought to determine the underlying mechanisms of action.
Methods: LPS was prepared from lyophilized P. intermedia ATCC 25611 cells by the standard hot phenol-water method. Culture supernatants were collected and assayed for IL-6. We used real-time polymerase chain reaction to detect IL-6 mRNA expression.
IκB-α degradation, nuclear translocation of NF-κB subunits, and STAT1 phosphorylation were characterized via immunoblotting.
DNA-binding of NF-κB was also analyzed.
Results: Curcumin strongly suppressed the production of IL-6 at both gene transcription and translation levels in P. intermedia LPS-activated RAW 264.7 cells. Curcumin did not inhibit the degradation of IκB-α induced by P. intermedia LPS. Curcumin blocked NF-κB signaling through the inhibition of nuclear translocation of NF-κB p50 subunit. Curcumin also attenuated DNA binding activity of p50 and p65 subunits and suppressed STAT1 phosphorylation.
Conclusions: Although further study is required to explore the detailed mechanism of action, curcumin may contribute to blockade of the host-destructive processes mediated by IL-6 and appears to have potential therapeutic values in the treatment of inflammatory periodontal disease.
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참고문헌 (23)
참고문헌 신청
Choi EY / 2011 / Melatonin inhibits Prevotella intermedia lipopolysaccharide-induced production of nitric oxide and interleukin-6 in murine macrophages by suppressing NF-κB
Chung CP / 1983 / Bacterial IgG and IgM antibody titers in acute necrotizing ulcerative gingivitis / J Periodontol 54 : 557 ~ 562
Geivelis M / 1993 / Measurements of interleukin-6 in gingival crevicular fluid from adults with destructive periodontal disease / J Periodontol 64 : 980 ~ 983
Hamada S / 1990 / Lipopolysaccharides of oral anaerobes associated with chronic inflammation: chemical and immunomodulating properties / Int Rev Immunol 6 : 247 ~ 261
Hashimoto M / 2003 / Chemical structure and immunobiological activity of lipid A from Prevotella intermedia ATCC 25611 lipopolysaccharide / FEBS Lett 543 : 98 ~ 102
Chung CP / 1983 / Bacterial IgG and IgM antibody titers in acute necrotizing ulcerative gingivitis / J Periodontol 54 : 557 ~ 562
Geivelis M / 1993 / Measurements of interleukin-6 in gingival crevicular fluid from adults with destructive periodontal disease / J Periodontol 64 : 980 ~ 983
Hamada S / 1990 / Lipopolysaccharides of oral anaerobes associated with chronic inflammation: chemical and immunomodulating properties / Int Rev Immunol 6 : 247 ~ 261
Hashimoto M / 2003 / Chemical structure and immunobiological activity of lipid A from Prevotella intermedia ATCC 25611 lipopolysaccharide / FEBS Lett 543 : 98 ~ 102
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