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학술저널
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대한치주과학회 Journal of Periodontal & Implant Science Journal of Periodontal & Implant Science 제43권 제4호
발행연도
2013.1
수록면
168 - 176 (9page)

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Purpose: The purpose of the current study was to examine the effect of dexamethasone (Dex) at various concentrations on the apoptosis and mineralization of human periodontal ligament (hPDL) cells . Methods: hPDL cells were obtained from the mid-third of premolars extracted for orthodontic reasons, and a primary culture of hPDL cells was prepared using an explant technique. Groups of cells were divided according to the concentration of Dex (0, 1, 10, 100, and 1,000 nM). A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed for evaluation of cellular viability, and alkaline phosphatase activity was examined for osteogenic differentiation of hPDL cells. Alizarin Red S staining was performed for observation of mineralization, and real-time polymerase chain reaction was performed for the evaluation of related genes. Results: Increasing the Dex concentration was found to reduce cellular viability, with an increase in alkaline phosphatase activity and mineralization. Within the range of Dex concentrations tested in this study, 100 nM of Dex was found to promote the most vigorous differentiation and mineralization of hPDL cells. Dex-induced osteogenic differentiation and mineralization was accompanied by an increase in the level of osteogenic and apoptosis-related genes and a reduction in the level of antiapoptotic genes. The decrease in hPDL cellular viability by glucocorticoid may be explained in part by the increased prevalence of cell apoptosis, as demonstrated by BAX expression and decreased expression of the antiapoptotic gene, Bcl-2. Conclusions: An increase in hPDL cell differentiation rather than cellular viability at an early stage is likely to be a key factor in glucocorticoid induced mineralization. In addition, apoptosis might play an important role in Dex-induced tissue regeneration;however, further study is needed for investigation of the precise mechanism.
#Apoptosis #Cell differentiation #Cell survival #Dexamethasone #Periodontal ligament.

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참고문헌 (23)

참고문헌 신청
Abe Y / 2000 / Development of mineralized nodules in fetal rat mandibular osteogenic precursor cells : requirement for dexamethasone but not for beta-glycerophosphate / Calc google schola Bartold PM / 2006 / Stem cells and periodontal regeneration / Periodontol 2000 40 : 164 ~ 172 google schola Bellows CG / 1990 / Determination of the capacity for proliferation and differentiation of osteoprogenitor cells in the presence and absence of dexamethasone / Dev Biol 140 google schola Bligh ME / 1993 / Aldosterone diurnal rhythm in the rat: a question of cross-reactivity? / Physiol Behav 53 : 845 ~ 848 google schola Chung CH / 1992 / Mechanism of action of beta-glycerophosphate on bone cell mineralization / Calcif Tissue Int 51 : 305 ~ 311 google schola

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