지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Objectives : Recent etiological studies show that oxidative stress might play a major role in the diabetes and its complications. Mori Ramulus (MR) has been known to have antioxidative, anti-inflammatory and antidiabetic effects. The methanol extract of MR was tested for its effectiveness in LLC-PK_1 cells exposed to high glucose. Methods : The cytoprotective effect of MR was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The antioxidative effect was measured in terms of generation amount of ․O_2- by 2',7'-dichlorodihydrofluorescein diacetate (DCFDA), NO by 4,5-diaminofluorescein (DAF-2), ONOO- by dihydrorhodamine 123 (DHR 123) in the high glucose -treated LLC-PK_1 cells. Western blotting was performed using anti-AGE, anti-RAGE, anti-MAPKs(ERK1/2, JNK, p38), anti-PI3K, anti-Akt, and anti-NF-κB (p50, p65) respectively. Results : MR extract reduced cell death and inhibited the generation of ․O_2-, NO, ONOO- in the high glucose-treated LLC-PK_1 cells. MR inhibited the expression of AGE, RAGE, MAPKs, PI3K, and Akt by means of decreasing NF-κB activation. MR also inhibited NF-κB activation itself. Conclusions : These results indicate MR has cytoprotective, antioxidative, and anti-inflammatory effects. Therefore it is suggested that MR might prevent and cure diabetes and its complications.
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참고문헌 (33)
참고문헌 신청
Cathcart R / 1983 / Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein fluorescent assay / Anal Biochem 134 : 111 ~ 116
Gorogawa S / 2002 / Probucol preserves pancreatic beta-cell function through reduction of oxidative stress in type 2 diabetes / Diabetes Res Clin Pract 57 (1) : 1 ~ 10
Kaneto H / 2002 / Involvement of c-Jun N-terminal kinase in oxidative stress-mediated suppression of insulin gene expression / J Biol Chem 277 (33) : 30010 ~ 30018
Kim HY / 2000 / Oxidative stress and atherosclerosis / 대한내분비학회지 15 (1) : 1 ~ 14
Kim OG / 2004 / Effects of the Mori folium extract in streptozotocin-induced diabetic rats / 석사 / 동국대학교 대학원
Gorogawa S / 2002 / Probucol preserves pancreatic beta-cell function through reduction of oxidative stress in type 2 diabetes / Diabetes Res Clin Pract 57 (1) : 1 ~ 10
Kaneto H / 2002 / Involvement of c-Jun N-terminal kinase in oxidative stress-mediated suppression of insulin gene expression / J Biol Chem 277 (33) : 30010 ~ 30018
Kim HY / 2000 / Oxidative stress and atherosclerosis / 대한내분비학회지 15 (1) : 1 ~ 14
Kim OG / 2004 / Effects of the Mori folium extract in streptozotocin-induced diabetic rats / 석사 / 동국대학교 대학원
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