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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 565 - 583 (19page)
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Objectives : This study investigated the biochemical mechanisms of anti-proliferative and pro-apoptotic effects of the water extract of Dan-Seon-Tang (DST) in human leukemia U937 cells.
Methods : U937 cells were exposed to DST and growth inhibition was measured by MTT assay.
Results : Exposure of U937 cells to DST resulted in the growth inhibition in a concentration-dependent manner. This inhibitory effect was associated with morphological changes and apoptotic cell death such as formation of apoptotic bodies, increased populations of apoptotic-sub G1 phase and induction of DNA fragmentation. The induction of apoptotic cell death in U937 cells by DST was associated with up-regulation of death receptor 4 (DR4) and down-regulation of Bid, surviving and cellular inhibition of apoptosis protein-2 (cIAP-2) expression. DST treatment also induced the proteolytic activation of caspase-3, caspase-8 and caspase-9, and a concomitant degradation of caspase-3 substrate proteins such as poly (ADP-ribose) polymerase (PARP), phospholipase (PLC)-γ1, β-catenin and DNA fragmentation factor 45/inhibotor of caspase activated DNAse (DFF45/ICAD). Furthermore, apoptotic cell death by DST was significantly inhibited by caspase-3 specific inhibitor z-DEVD-fmk, demonstrating the important role of caspase-3.
Conclusions : These findings suggest that herb prescription DST may be a potential chemotherapeutic agent for the control of human leukemia U937 cells; further study is needed to identify the active compounds.
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참고문헌 (55)
참고문헌 신청
Rhee SG / 1997 / Regulation of phosphoinositide -specific phospholipase C isozymes / J Biol Chem 272:15045~15048
Kamat A / 1997 / Phospholipase C-gamma1: regulation of enzyme function and role in growth factor-dependent signal transduction / Cytokine Growth Factor Rev 8:109~117
Nishizuka Y / 2003 / Early studies of protein kinase C: a historical perspective / Methods Mol Biol 233:9~18
Berridge MJ / 1984 / Inositol trisphosphate, a novel second messenger in cellular signal transduction / Nature 312:315~321
Myklebust JH / 2002 / Activation of phosphatidylinositol 3-kinase is important for erythropoietin-induced erythropoiesis from CD34(+) hematopoietic progenitor cells / Exp Hematol 30:990~1000
Kamat A / 1997 / Phospholipase C-gamma1: regulation of enzyme function and role in growth factor-dependent signal transduction / Cytokine Growth Factor Rev 8:109~117
Nishizuka Y / 2003 / Early studies of protein kinase C: a historical perspective / Methods Mol Biol 233:9~18
Berridge MJ / 1984 / Inositol trisphosphate, a novel second messenger in cellular signal transduction / Nature 312:315~321
Myklebust JH / 2002 / Activation of phosphatidylinositol 3-kinase is important for erythropoietin-induced erythropoiesis from CD34(+) hematopoietic progenitor cells / Exp Hematol 30:990~1000
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