지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 550 - 564 (15page)
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초록· 키워드
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Objectives : Oxidative stress seems to play a major role in mechanisms by which ethanol causes liver injury. Previous studies have shown that treatment with Yinjinchunggan-tang (Yinchenqinggan-tang, YJCGT) has protective effects on alcoholic liver disease. The aim of this study was to investigate the protective effects of YJCGT on alcohol-induced oxidative stress.
Materials and Methods : In vitro, we evaluated the inhibitory activities of YJCHT on DPPH(1,1-diphenyl-2-picryl-hydrazyl), xanthine oxidase, trypsin, and hyaluronidase. In a cell culture model, we measured cell viability and proliferation, and the activities of superoxide dismutase (SOD), and catalase (CAT) after YJCGT treatment in C34 and E47 cell lines, and HepG2 cells transfected with/ without cytochrome P450ⅡE1 (CYP2E1) gene. In vivo, we estimated serum level of hepatic biochemical markers, and alcohol concentration in the blood.
Results : YJCGT showed significant free radical scavenging activity against DPPH and xanthine oxidase and decreased hyaluronidase activity effectively in vitro. YJCGT also increased cell viability, and proliferation in C34 and in E47 cell lines, and increased activities of superoxide dismutase, and catalase in C34 and in E47 cell lines. YJCGT reduced serum AST, LDH, and total cholesterol level in some of the results, and reduced blood alcohol concentration in vivo, as well.
Conclusions : This study suggests that YJCGT has protective effects on oxidative stress by inhibiting alcohol-induced suppression of antioxidant enzyme activities.
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참고문헌 (35)
참고문헌 신청
2009 국민건강통계 / 2010 / 2009 국민건강통계. 국민건강영양조사 제4기 3차년도 / 보건복지부, 질병관리본부 : 22 ~ 23
Anson ML / 1938 / The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin / J Gen Physiol 22 : 79 ~ 89
Bergmeyer HU / 1974 / Methods of Enzymatic Analysis(Bergmeyer, H.U., ed.) / Verlag Chemie, Weinheim, and Academic Press : 944 ~
Blois MS / 1958 / Antioxidant determinations by the use of a stable free radical / Nature 181 : 1199 ~ 1200
Castillo T / 1992 / Role of cytochrome P-450 2E1 in ethanol-carbon tetrachloride and iron-dependent microsomal lipid peroxidation / Hepatology 16 : 992 ~ 996
Anson ML / 1938 / The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin / J Gen Physiol 22 : 79 ~ 89
Bergmeyer HU / 1974 / Methods of Enzymatic Analysis(Bergmeyer, H.U., ed.) / Verlag Chemie, Weinheim, and Academic Press : 944 ~
Blois MS / 1958 / Antioxidant determinations by the use of a stable free radical / Nature 181 : 1199 ~ 1200
Castillo T / 1992 / Role of cytochrome P-450 2E1 in ethanol-carbon tetrachloride and iron-dependent microsomal lipid peroxidation / Hepatology 16 : 992 ~ 996
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