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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 286 - 292 (7page)
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초록· 키워드
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We have constructed a cDNA library using brain samples of olive flounder Paralichthys olivaceus. Here, we described the study on gene identification by screening 356 clones from the brain cDNA library of olive flounder. Here, we screened 356 clones from the library to identify genes. Of these, 176 (49.5%) were identified as orthologs of known genes from olive flounder and other organisms. Among the 176 EST clones, 33 (18.7%) represented 11 unique genes that are identical to expressed sequence tags (ESTs) reported for olive flounder, and 120 (68.2%) represented 102 unique genes known from other organisms. The percentage of unknown genes (50.5%) is higher than in other olive flounder cDNA libraries (Lee et al., 2003, 2006, 2007), reflecting the high complexity of brain tissue. Further studies of expression characterization and developmental behavior related to these genes should provide useful insight into the physiological functions of the brain in olive flounder.
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등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Adams MD / 1991 / Complementary DNA se-uenceing: expressed sequence tags and human genome project / Science 252 : 1651 ~ 1656
Aoki T / 1999 / Sequences of 596 cDNA (565,977 bp) clones of Japanese flounder (Paralichthys olivaceus) leukocytes infected with hirame rhabdovirus / Mar Biotechnol 1 : 477 ~
Chen SL / 2004 / Analysis of immunerelevant genes expressed in red sea bream (Chrysophrys major) spleen / Aquaculture 240 : 115 ~ 130
Chomczynski P / 1987 / Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction / Analyt Biochem 162 : 156 ~ 159
Davey GC / 2001 / A survey of genes in Atlantic salmon (Salmo salar) as identified by expressed sequence tags / Gene 263 : 121 ~ 130
Aoki T / 1999 / Sequences of 596 cDNA (565,977 bp) clones of Japanese flounder (Paralichthys olivaceus) leukocytes infected with hirame rhabdovirus / Mar Biotechnol 1 : 477 ~
Chen SL / 2004 / Analysis of immunerelevant genes expressed in red sea bream (Chrysophrys major) spleen / Aquaculture 240 : 115 ~ 130
Chomczynski P / 1987 / Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction / Analyt Biochem 162 : 156 ~ 159
Davey GC / 2001 / A survey of genes in Atlantic salmon (Salmo salar) as identified by expressed sequence tags / Gene 263 : 121 ~ 130
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