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- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
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The objective of this study was to determine a simple and reliable ploidy identification protocol for the rainbow trout (RT), Oncorhynchus mykiss, in the field condition. To evaluate the ploidy level and compare different detection protocols, triploid RT and gynogenesis were induced by UV irradiation and/or heat shock. The hatching rate at day 30 was 85.2% and the survival rate at day 90 was 69.4% (fingerling). The sex ratio of female RT was 93.75% in the gynogenesis group, illustrating that the UV irradiation inactivated the sperm DNA. The hatching rate and survival rate were 82.0 and 74.7%, respectively, in the triploid-induced group. The triploid induction rate by heat shock procedure was 73.9%. Cytogenetic protocols for ploidy identification such as chromosome counting, erythrocyte nuclear size comparison, and analysis of nucleolar organizing regions (NORs) by silver staining were compared.
Silver nitrate staining showed the greatest success rate (22/23 and 32/32 for the triploid-induced group and gynogenesis group, respectively), followed by erythrocyte nuclear size comparison (16/23 and 19/32 for the triploid-induced group and gynogenesis group, respectively) and, lastly, chromosome preparation (2/23 and 6/32 for the triploid-induced group and gynogenesis group, respectively) with the lowest success rate. Based on our findings, silver staining for RT ploidy identification is speculated to be highly applicable in a wide range of research conditions, due to its cost-effectiveness and simplicity compared to other numerous ploidy detection protocols.
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참고문헌 (26)
참고문헌 신청
Alcantar-Vazquez JP / 2008 / Induction of triploidy in spotted sand bass(Paralabrax maculatofasciatus Steindachner, 1868)by cold shock / Aquac Res 39 : 59 ~ 63
Alcantar-Vazquez JP. / 2016 / Fisiologia de los peces triploides / Lat Am J Aquat Res 44 : 1 ~ 15
Barch MJ / 1997 / The AGT cytogenetic laboratory manual / Raven : 25 ~ 50
Cal RM / 2006 / Growth and gonadal development in diploid and triploid turbot(Scophthalmus maximus) / Aquaculture 251 : 99 ~ 108
Carman O / 1992 / Variation in the maximum number of nucleoli in diploid and triploid common carp / Nippon Suisan Gakkai Shi 58 : 2303 ~ 2309
Alcantar-Vazquez JP. / 2016 / Fisiologia de los peces triploides / Lat Am J Aquat Res 44 : 1 ~ 15
Barch MJ / 1997 / The AGT cytogenetic laboratory manual / Raven : 25 ~ 50
Cal RM / 2006 / Growth and gonadal development in diploid and triploid turbot(Scophthalmus maximus) / Aquaculture 251 : 99 ~ 108
Carman O / 1992 / Variation in the maximum number of nucleoli in diploid and triploid common carp / Nippon Suisan Gakkai Shi 58 : 2303 ~ 2309
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