지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 43 - 54 (12page)
이용수
초록· 키워드
오류제보하기
Gene structure of copper/zinc-superoxide dismutase (Cu/Zn-SOD; sod1) was characterized in Hemibarbus mylodon (Teleostei; Cypriniformes), an endangered freshwater fish species in Korean peninsula. Full-length cDNA of H. mylodon SOD1 consisted of a 796-bp open reading frame sequence encoding 154 amino acids, and the deduced polypeptide sequence shared high sequence homology with other orthologs, particularly with regard to metal-coordinating ligands.
Genomic structure of the H. mylodon sod1 gene (hmsod1; 1,911 bp from the ATG start codon to the stop codon) was typical quinquepartite (i.e., five exons interrupted by four introns); the lengths of the exons were similar among species belonging to various taxonomic positions. The molecular phylogeny inferred from sod1 genes in the teleost lineage was in accordance with the conventional taxonomic assumptions. 5'-flanking upstream region of hmsod1, obtained using the genome walking method, contained typical TATA and CAAT boxes. It also showed various transcription factor binding motifs that may be potentially involved in stress/immune response (e.g., sites for activating proteins or nuclear factor kappa B) or metabolism of xenobiotic compounds (e.g., xenobiotic response element; XRE). The hmsod1 transcripts were ubiquitously detected among tissues, with the liver and spleen showing the highest and lowest expression,respectively. An experimental challenge with Edwardsiella tarda revealed significant upregulation of the hmsod1 in kidney (4.3-fold) and spleen (3.1-fold), based on a real-time RT-PCR assay. Information on the molecular characteristics of this key antioxidant enzyme gene could be a useful basis for a biomarker-based assay to understand cellular stresses in this endangered fish species.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Afonso V / 2006 / Tumor necrosis factor-α down regulates human Cu/Zn superoxide dismutase 1 promoter via JNK/AP-1 signaling pathway / Free Rad Biol Med 41 : 709 ~ 721
Almeida JA / 2002 / The use of the oxidative stress responses as biomarkers in Nile tilapia(Oreochromis niloticus)exposed to in vivo cadmium contamination / Environ Intl 27 :
Cheng W / 2006 / Molecular cloning and characterization of copper/zinc superoxide dismutase(Cu,Zn-SOD)from the giant freshwater prawn Macrobarachium rosenbergii / Fish Shellf
Cho YS / 2006 / Differential expression of Cu/Zn superoxide dismutase mRNA during exposures to heavy metals in rockbream (Oplegnathus fasciatus) / AQUACULTURE 253 : 667 ~ 679
Cho YS / 2008 / Gene structure and expression of metallothionein during metal exposures in Hemibarbus mylodon / ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 71 : 125 ~ 137
Almeida JA / 2002 / The use of the oxidative stress responses as biomarkers in Nile tilapia(Oreochromis niloticus)exposed to in vivo cadmium contamination / Environ Intl 27 :
Cheng W / 2006 / Molecular cloning and characterization of copper/zinc superoxide dismutase(Cu,Zn-SOD)from the giant freshwater prawn Macrobarachium rosenbergii / Fish Shellf
Cho YS / 2006 / Differential expression of Cu/Zn superoxide dismutase mRNA during exposures to heavy metals in rockbream (Oplegnathus fasciatus) / AQUACULTURE 253 : 667 ~ 679
Cho YS / 2008 / Gene structure and expression of metallothionein during metal exposures in Hemibarbus mylodon / ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 71 : 125 ~ 137
이 논문의 저자 정보
최근 본 자료
댓글(0)
0