지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 177 - 185 (9page)
이용수
초록· 키워드
오류제보하기
The development of species-specific fish cell lines has become a valuable tool for biological research. In recent years, marine medaka Oryzias dancena has been recognized as a good experimental model fish but there are no reports of establishment of cell lines from this fish. In this study, two cell lines from O. dancena blastula embryos were established from 41 total trials (4.9%). The two cell lines displayed typical in vitro morphology and have been cultured for >121 passages, which corresponds to 293 days. The doubling times of the cell lines were 29.84 and 28.59 h, respectively, and both possessed the potential to expand in a clonal manner, albeit with significant differences between the two cell lines. The absence of any of the four main medium supplements; i.e., fish serum, fetal bovine serum, basic fibroblast growth factor, and medaka embryo extract, significantly inhibited growth. The proportion of cells possessing normal chromosome number was 45% and 46.7% of the cell lines, respectively. Taken together, two cell lines that proliferate continuously were established from marine medaka and these cell lines may provide a basic tool for characterizing the unique features of this fish species.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Bejar J / 2002 / An ES-like cell line from the marine fish Sparus aurata: characterization and chimaera production / Transgenic Res 11 : 279 ~ 289
Boilly B / 2000 / FGF signals for cell proliferation and migration through different pathways / Cytokine Growth Factor Rev 11 : 295 ~ 302
Bols NC / 1994 / Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss (Walbaum), gills / J Fish Dis 17 : 601 ~ 611
Collodi P / 1990 / Mitogenic activity from trout embryos / Proc Natl Acad Sci U S A 87 : 3498 ~ 3502
Dalle Nogare DE / 2009 / G2 acquisition by transcription-independent mechanism at the zebrafish midblastula transition / Dev Biol 326 : 131 ~ 142
Boilly B / 2000 / FGF signals for cell proliferation and migration through different pathways / Cytokine Growth Factor Rev 11 : 295 ~ 302
Bols NC / 1994 / Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss (Walbaum), gills / J Fish Dis 17 : 601 ~ 611
Collodi P / 1990 / Mitogenic activity from trout embryos / Proc Natl Acad Sci U S A 87 : 3498 ~ 3502
Dalle Nogare DE / 2009 / G2 acquisition by transcription-independent mechanism at the zebrafish midblastula transition / Dev Biol 326 : 131 ~ 142
이 논문의 저자 정보
최근 본 자료
댓글(0)
0