지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 1 - 8 (8page)
이용수
초록· 키워드
오류제보하기
To culture germline stem cells in vitro, establishment of the cell lines that can be used as the feeder cells is a prerequisite. In this study, we tried to establish gonad-derived cell lines in Siberian sturgeon (Acipenser baerii). Five 1-year-old A. baerii were used as a donor of gonad tissues, and gonad-dissociated cells were cultured in vitro.
Subsequently, determination of growth conditions, long-term culture, characterization, and cryopreservation of the cell lines were also conducted. Five gonad-derived cell lines were stably established and cultured continuously over at least the 73th passage and 402 culture days under the media containing 20 % fetal bovine serum at 28 °C. All cell lines consisted of two main cell types based on morphology even if the ratio of the two cell types was different depending on cell lines. Despite long-term culture, all cell lines maintained diploid DNA contents and expression of several genes that are known to express in the A. baerii gonad. After freezing and thawing of the cell lines, post-thaw cell viabilities between 57.6 and 92.9 % depending on cell lines were indentified, suggesting that stable cryopreservation is possible. The results and the cell lines established in this study will contribute to the development of an in vitro system for A. baerii germline stem cell culture.
목차
등록된 정보가 없습니다.
참고문헌 (43)
참고문헌 신청
Amit M / 2003 / Human feeder layers for human embryonic stem cells / Biol Reprod 68:2150~2156
Aponte PM / 2008 / Propagation of bovine spermatogonial stem cells in vitro / Reproduction 136:543~557
Berbejillo J / 2012 / Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii / Mol Reprod Dev 79:504~516
Brinster RL / 2007 / Male germline stem cells : from mice to men / Science 316:404~405
Evans MJ / 1981 / Establishment in culture of pluripotential cells from mouse embryos / Nature 292:154~156
Aponte PM / 2008 / Propagation of bovine spermatogonial stem cells in vitro / Reproduction 136:543~557
Berbejillo J / 2012 / Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii / Mol Reprod Dev 79:504~516
Brinster RL / 2007 / Male germline stem cells : from mice to men / Science 316:404~405
Evans MJ / 1981 / Establishment in culture of pluripotential cells from mouse embryos / Nature 292:154~156
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Anesthetic effects and physiological responses of lidocaine-HCl in Siberian sturgeon, Acipenser baerii
한국수산과학회 양식분과 학술대회
2016 .05
-
EFFECTS OF VITAMIN E DEFICIENCY ON DIETARY VITAMIN C REQUIREMENT IN SIBERIAN STURGEON (Acipenser baerii)
한국수산과학회 양식분과 학술대회
2015 .05
-
Generation of interspecific hybrid genotype between Siberian sturgeon (Acipenser baerii) and Russian sturgeon (A. gueldenstaedii)
한국수산과학회 양식분과 학술대회
2016 .05
-
Studies on Conditioned Media in Human Cells: Evaluation Using Various Cell and Culture Conditions, Animal Disease Models
한국수정란이식학회지
2018 .01
-
Methodologies for Cryopreservation of Mammalian Germline Cells and Tissues
한국동물번식학회지
2017 .01
-
Isolation and in vitro culture of primary cell populations derived from ovarian tissues of the rockfish, Sebastes schlegeli
Fisheries and Aquatic Sciences
2016 .01
-
Factors Affecting Primary Cultures of Haliotis discus hannai Ovary-dissociated Cells and General Culture Aspects
Fisheries and Aquatic Sciences
2015 .01
-
Establishment of conditions for long-term maintenance of primary embryonic cell cultures from olive flounder Paralichthys olivaceus
한국수산과학회 양식분과 학술대회
2021 .04
-
Glyphosate influences cell proliferation in cultured human kidney cells
한국농약과학회 학술발표대회 논문집
2018 .10
-
Anesthetic Effects and Physiological Responses of Lidocaine-HCl in Siberian Sturgeon, Acipenser baerii
수산해양교육연구
2019 .04
-
Effect of adipose tissue-derived exosomes on the activities of NK cells and CD8 T cells against Panc02 cells
한국식품영양과학회 학술대회발표집
2022 .10
-
Development of New Methods for Isolation, Preservation of Adipose-Derived Stem Cells and Differentiation of Dermal Papilla Cells from Adipose-Derived Stem Cells
한국식품영양과학회 학술대회발표집
2022 .10
-
Principal protocols for the processing of cultured meat
한국축산학회지
2021 .01
-
Establishment and characterization of homogenous cell lines from Acipenser baerili head kidney
한국수산과학회 양식분과 학술대회
2016 .05
-
Establishment of Optimal Culture Methods for Obtaining Protein from Animal Muscle Stem Cells
한국식품영양과학회 학술대회발표집
2024 .10
-
Influence of co-culturing muscle satellite cells with preadipocytes on the differentiation of adipocytes and muscle cells isolated from Korean native cattle
Korean Journal of Agricultural Science
2018 .12
-
배아추출물 공여 배아의 발생단계가 바다송사리(Oryzias dancena) 배아 줄기세포 유사세포의 배양에 미치는 영향
한국수산과학회지
2017 .04
-
Effect of Compound K on the Accumulation of Lipids in 3T3-L1 Cells and HepG2 Cells
한국식품영양과학회 학술대회발표집
2023 .10
-
Dioscin reduces cell proliferation through cell cycle arrest by modulating AKT/p53/p21 signaling pathways in breast cancer stem-like cells
한국식품영양과학회 학술대회발표집
2019 .10
-
Ursolic acid Decreases the Proliferation of MCF-7 Cell-derived Breast Cancer Stem-like Cells by Modulating the ERK and PI3K/AKT Signaling Pathways
한국식품영양과학회 학술대회발표집
2021 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0