지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 501 - 505 (5page)
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Background and Objectives Mucus hypersecretion in the airway may lead to increased frequency and duration of infection, declined lung function, and increased morbidity and mortality in inflammatory respiratory diseases. Udenafil, a phosphodiesterase (PDE) 5 inhibitor, is an oral medication for erectile dysfunction. Recent studies show that PDE5 inhibitor has various anti-inflammatory properties. However, the effect of udenafil on mucus secretion in human airway epithelial cells is unclear. Therefore, the effect and brief signaling pathway of udenafil on MUC5B expression were investigated in human airway epithelial cells.
Materials and Method We analyzed the effects and brief signaling pathway of udenafil on the lipopolysaccharide (LPS) induced MUC5B expression in mucin-producing NCI-H292 epithelial cells using reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, and immunoblot analysis.
Results Udenafil attenuated the LPS-induced MUC5B mRNA expressions and glycoprotein production in NCI-H292 epithelial cells. It also attenuated LPS-induced toll like receptor 4 (TLR4) mRNA expression and phosphorylation of extracellular regulated kinase1/2 (ERK1/2) and p38 in NCI-H292 epithelial cells.
Conclusion These results suggest that udenafil attenuates the LPS induced MUC5B expression via TLR4, ERK1/2 and p38 mitogen activated protein kinase (MAPK) pathway in human airway epithelial cells, and that it could be a novel therapeutic agent for controlling chronic airway disease.
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참고문헌 (20)
참고문헌 신청
Ali MS / 2007 / Upper airway mucin gene expression: a review / Laryngoscope 117 (5) : 932 ~ 938
Bae CH / 2011 / AMPK induces MUC5B expression via p38 MAPK in NCI-H292 airway epithelial cells / Biochem Biophys Res Commun 409 (4) : 669 ~ 674
Bae CH / 2013 / Insulin-like growth factor-1 induces MUC8 and MUC5B expression via ERK1 and p38 MAPK in human airway epithelial cells / Biochem Biophys Res Commun 430 (2) :
Borchers MT / 1999 / Regulation of human airway mucins by acrolein and inflammatory mediators / Am J Physiol 276 (4 Pt 1) : L549 ~ L555
Dabbagh K / 1999 / IL-4 induces mucin gene expression and goblet cell metaplasia in vitro and in vivo / J Immunol 162 (10) : 6233 ~ 6237
Bae CH / 2011 / AMPK induces MUC5B expression via p38 MAPK in NCI-H292 airway epithelial cells / Biochem Biophys Res Commun 409 (4) : 669 ~ 674
Bae CH / 2013 / Insulin-like growth factor-1 induces MUC8 and MUC5B expression via ERK1 and p38 MAPK in human airway epithelial cells / Biochem Biophys Res Commun 430 (2) :
Borchers MT / 1999 / Regulation of human airway mucins by acrolein and inflammatory mediators / Am J Physiol 276 (4 Pt 1) : L549 ~ L555
Dabbagh K / 1999 / IL-4 induces mucin gene expression and goblet cell metaplasia in vitro and in vivo / J Immunol 162 (10) : 6233 ~ 6237
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