기니아피그 와우신경절에서 배양된 성체 신경줄기세포의  유세포분석법을 이용한 분석

김영하; 김창현; 박경호; 박시내; 최미영; 홍옥기; 김지홍; 조상재; 김정현; 장기홍; 여상원

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자료유형: 학술저널

저자정보: 김영하 김창현 박경호 박시내 최미영 홍옥기 김지홍 조상재 김정현 장기홍 여상원

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제52권 제2호

발행연도: 2009.1

수록면: 129 - 133 (5page)

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연구배경

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연구방법

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Background and Objectives：To make stem cell therapy successful as one of treatment options for sensorineural hearing loss, it is essential to culture and obtain sufficient amounts of adult neural stem cells, as well as separating them from adult auditory organs. This study was designed to investigate the proportion of cultured adult neural stem cells and its differentiated cells from guinea pig spiral ganglion. Materials and Method：The spiral ganglions from guinea pigs of 3-6 month age were obtained. The tissues were digested with 0.25 % trypsin and 10 mg/mL of DNase I, cells were then cultured with neurobasal medium (DMEM/ F12 containing B27 supplement, L-glutamin, gentamycin) and added with 20 ng/mL of epidermal growth factor and 10 ng/mL of fibroblast growth factor. After 3 passages of culture, neural stem cells and differentiated cells were analyzed with the flow cytometric method. Results：We concluded that neural stem cells were successfully cultured from spiral ganglions and these cells were in process of differentiation into neurons and Schwann cells. The results of flow cytometric analysis of cells in culture medium showed that 1.7% of cells (cell count of 24,300) expressed nestin, 3.45% polysialylated neural cell adhesion molecule, 7.19% (cell count of 66,300) neural cell adhesion molecule, and 3.57% β III tubulin. Conclusion：Though obtaining adult neural stem cells from adult spiral ganglion was successful, the cell count was small. Further studies on the subject of making proper culture medium are needed to obtain adequate amounts of adult neural stem cells.

#줄기세포·와우신경절·유세포분석법 #Stem cell·Spiral ganglion·Flow cytometry

참고문헌 (17)

참고문헌 신청

Bodine DM / 1991 / Effects of hematopoietic growth factors on the survival of primitive stem cells in liquid suspension culture / Blood 78 (3) : 914 ~ 920

Gritti A / 1996 / Multipotential stem cells from the adult mouse brain proliferate and self-renew in response to basic fibroblast growth factor / J Neurosci 16 (3) : 1091 ~ 1

Hu Z / 2005 / Survival and neural differentiation of adult neural stem cells transplanted into the mature inner ear / Exp Cell Res 302 (1) : 40 ~ 47

Li H / 2003 / Pluripotent stem cells from the adult mouse inner ear / Nat Med 9 (10) : 1293 ~ 1299

Lou X / 2007 / Multipotent stem cells from the young rat inner ear / Neurosci Lett 416 (1) : 28 ~ 33

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