The Presence of Neural Stem Cells and Changes in Stem Cell-Like Activity With Age in Mouse Spiral Ganglion Cells In Vivo and In Vitro

Byoung-San Moon; Aswathy Ammothumkandy; Naibo Zhang; Lei Peng; Albina Ibrayeva; Maxwell Bay; Athira Pratap; 박홍주; Michael Anthony Bonaguidi; Wange Lu

추천

검색

자료유형: 학술저널

저자정보: Byoung-San Moon Aswathy Ammothumkandy Naibo Zhang Lei Peng Albina Ibrayeva Maxwell Bay Athira Pratap 박홍주 Michael Anthony Bonaguidi Wange Lu

저널정보: 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제11권 제4호

발행연도: 2018.1

수록면: 224 - 232 (9page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Objectives. Spiral ganglion neurons (SGNs) include potential endogenous progenitor populations for the regeneration of the peripheral auditory system. However, whether these populations are present in adult mice is largely unknown. We examined the presence and characteristics of SGN-neural stem cells (NSCs) in mice as a function of age. Methods. The expression of Nestin and Ki67 was examined in sequentially dissected cochlear modiolar tissues from mice of different ages (from postnatal day to 24 weeks) and the sphere-forming populations from the SGNs were isolated and differentiated into different cell types. Results. There were significant decreases in Nestin and Ki67 double-positive mitotic progenitor cells in vivo with increasing mouse age. The SGNs formed spheres exhibiting self-renewing activity and multipotent capacity, which were seen in NSCs and were capable of differentiating into neuron and glial cell types. The SGN spheres derived from mice at an early age (postnatal day or 2 weeks) contained more mitotic stem cells than those from mice at a late age. Conclusion. Our findings showed the presence of self-renewing and proliferative subtypes of SGN-NSCs which might serve as a promising source for the regeneration of auditory neurons even in adult mice.

참고문헌 (26)

참고문헌 신청

Kujawa SG / 2009 / Adding insult to injury: cochlear nerve degeneration after “temporary” noise-induced hearing loss / J Neurosci 29(45):14077~14085

Hoeffding V / 1988 / Changes with age in the morphology of the cochlear nerve in rats: light microscopy / J Comp Neurol 276(4):537~546

Roehm PC / 2005 / Strategies to preserve or regenerate spiral ganglion neurons / Curr Opin Otolaryngol Head Neck Surg 13(5):294~300

Shi F / 2013 / Prospects for replacement of auditory neurons by stem cells / Hear Res 297:106~112

Reyes JH / 2008 / Glutamatergic neuronal differentiation of mouse embryonic stem cells after transient expression of neurogenin 1 and treatment with BDNF and GDNF: in vitro and in vivo studies / J Neurosci 28(48):12622~12631

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!