지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 37 - 42 (6page)
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The cells are concentrated approximately 20-fold by cytocentrifugation. This study evaluated the nucleated cell number for cells recovered on slide by using Cytospin-3 (Thermo Shandon Ltd. UK) and Cytopro-7620 (Wescor Inc., USA) cytocentrifuges to hematocytometer cell count of 0∼5 WBCs/μL of hematocytometer in the cerebrospinal fluid cell count. One hundred forty eight samples of 0∼5 WBCs/μL on hematocytometer, were cytocentrifuged by Cytospin-3 and Cytopro-7620 instruments. The nucleated cell number for cells recovered on slide was counted after Wright stain. The nucleated cell number for cells recovered on slide was 0∼40 cells in the 44 samples of 0 WBC/μL, and 3∼95 cells in the 31 samples of 1 WBC/μL. It was observed that the nucleated cell number for cells recovered on slide was 13∼100 cells in the 44 samples of 2 WBCs/μL, and more than 100 cells in the 29 samples of 3∼5 WBCs/μL, respectively. In addition, extremely normal lymphocyte, monocyte and polymorphonuclear neutrophil were observed in the 143 samples of 0∼5 WBCs/μL. Macrophage and eosinophil were also rarely observed. The nucleated cell number for cells recovered on slide was 20 cells, which were regarded as 1 WBC/μL in body fluid cell count. However, in this study, we made alterations to report nucleated cell percentage as 0% without preparing the cytocentrifuged slide at 0 WBC/μL by using the cell yield in a comparison between the value of 0∼5 WBCs/μL and nucleated cell number for cells recovered on slide.
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참고문헌 (27)
참고문헌 신청
Abbott BL / 2003 / Clinical significance of central nervous system involvement at diagnosis of pediatric acute myeloid leukemia: a single institution’s experience / Leukemia
Aulesa C / 2003 / Use of the Advia-120 hematology analyzer in the differential cytologic analysis of biological fluids (cerebrospinal, peritoneal, pleural, pericardial, synov
Bames PW / 2004 / An evaluation of the utility of performing body fluid counts on the Coulter LH-750 / Lab Hematol 10 : 127 ~ 131
Chapin-Robertson K / 1992 / Clinical and laboratory analysis of cytopsin-prepared Gram stains for recovery and diagnosis of from sterile body fluids / J Clin Microbiol 30 : 3
Chow G / 1984 / Lysis of erythrocytes and leukocytes in traumatic lumbar punctures / Arch Neurol 41 : 1084 ~ 1085
Aulesa C / 2003 / Use of the Advia-120 hematology analyzer in the differential cytologic analysis of biological fluids (cerebrospinal, peritoneal, pleural, pericardial, synov
Bames PW / 2004 / An evaluation of the utility of performing body fluid counts on the Coulter LH-750 / Lab Hematol 10 : 127 ~ 131
Chapin-Robertson K / 1992 / Clinical and laboratory analysis of cytopsin-prepared Gram stains for recovery and diagnosis of from sterile body fluids / J Clin Microbiol 30 : 3
Chow G / 1984 / Lysis of erythrocytes and leukocytes in traumatic lumbar punctures / Arch Neurol 41 : 1084 ~ 1085
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