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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 19 - 25 (7page)
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초록· 키워드
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Porcine reproductive and respiratory syndrome (PRRS) virus is the etiological agent of diseases characterized by reproductive losses in sows and respiratory disorders in piglets. The PRRS virus is a small enveloped virus containing a positive-sense, single-stranded RNA genome. In the present study, ORF6 gene of Korean PRRS virus isolate, CNV, was cloned and expressed in baculovirus expression system. The ORF6 gene and expressed protein in the recombinant virus were confirmed by PCR/indirect fluorescence
antibody (IFA) test and Western blotting, respectively. The recombinant protein with a molecular weight of approximately 24KDa was confirmed by Western blotting using His6 and PRRS virus-specific antiserum. Expressed ORF6 protein was applied for IFA to detect antibody against PRRS virus using field porcine sera. However, the sensitivity and specificity of developed IFA using expressed ORF6 protein were considerably low compared to those of commercial ELISA kit. This results suggest that IFA using expressed
ORF6 protein could not be used as a diagnostic test for PRRS virus infection without further improvements.
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등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Albina E / 1992 / An enzyme linked immunosorbent assay (ELISA) for the detection of antibody to porcine reproductive and respiratory syndrome virus / Ann Rec Vet 23 : 167 ~ 1
Bautista EM / 1998 / Structural polypeptides of the American (VR-2332) strain of porcine reproductive and respiratory syndrome virus / Arch Virol 141 : 357 ~ 1365
Cavanagh D / 1997 / Nidovirales: A new order comprising Coronaviridae and Arteriviridae / Arch Virol 142 : 629 ~ 633
Dea S / 2000 / Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome (PRRS) virus / Arch Virol 145 : 659 ~ 688
Houben S / 1995 / Comparative study of a blocking enzyme linked immunosorbent assay and the immunoperoxidase monolayer assay for the detection of antibody to porcine reproduc
Bautista EM / 1998 / Structural polypeptides of the American (VR-2332) strain of porcine reproductive and respiratory syndrome virus / Arch Virol 141 : 357 ~ 1365
Cavanagh D / 1997 / Nidovirales: A new order comprising Coronaviridae and Arteriviridae / Arch Virol 142 : 629 ~ 633
Dea S / 2000 / Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome (PRRS) virus / Arch Virol 145 : 659 ~ 688
Houben S / 1995 / Comparative study of a blocking enzyme linked immunosorbent assay and the immunoperoxidase monolayer assay for the detection of antibody to porcine reproduc
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돼지생식기호흡기증후군 바이러스의 Nucleocapsid 단백질의 세포 내 분포
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새로운 돼지질병 'PRRS' -세계의 연구진전 방향-
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