지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 215 - 222 (8page)
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초록· 키워드
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Species-specific TaqManⓇ probe-based real-time PCR assays were developed for detection of beef, pork, chicken, duck, goose and turkey. The primer and probe sets used in this study were designed to be complementary to fibroblast growth factor (FGF) for cattle and pig, mitochondrial NADH dehydrogenase (ND) subunit 3 and ND2 for chicken and duck, 12S rRNA for goose and turkey, respectively. As internal positive control we used conserved region in the ribosomal 18S RNA gene to ensure the accuracy of the detection of target DNA by real-time PCR. We confirmed that real-time PCR assays with the primer and probe sets were positive for cattle, pig and chicken intended target animal species with no cross-reactivity with other non-target animal species. Only ＞50 ng DNA of beef show cross-reactivity in the determination of duck. Using species-specific primer and probe sets, it was possible to detect amounts of 0.1 ng DNA of cattle and pig, 1.0 pg DNA of chicken, duck and turkey, and 0.1 pg DNA of goose for raw samples, respectively. The detection limits were 0.1 ng DNA of cattle, 1.0 ng DNA of pig and 1.0 pg DNA of chicken for DNA mixtures (beef, pork and chicken) extracted from heat-treated (121oC/5 min) meat samples. In conclusion, it can be suggested that the TaqManⓇ probe-based assay developed in this study might be a rapid and specific method for the identification of meat species in raw or cooked meat products.
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등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Ashoor SH. / 1988 / Liquid chromatographic quantitation of chicken and turkey in unheated chicken-turkey mixtures / J Assoc Off Anal Chem 71 : 403 ~ 405
Ballin NZ. / 2010 / Authentication of meat and meat products / Meat Science 86 : 577 ~ 587
Bottero MT. / 2011 / Animal species identification in food products : evolution of biomolecular methods. Vet J 190 : 34-38 / Vet J 190 : 34 ~ 38
Camma C. / 2012 / Development and validation of fast Real-Time PCR assays for species identification in raw and cooked meat mixtures / Food Control 23 : 400 ~ 404
Crockett AO. / 2001 / Fluorescein-labeled oligonucleotides for real-time PCR: using the inherent quenching of deoxyguanosine nucleotides / Anal Biochem 290 : 89 ~ 97
Ballin NZ. / 2010 / Authentication of meat and meat products / Meat Science 86 : 577 ~ 587
Bottero MT. / 2011 / Animal species identification in food products : evolution of biomolecular methods. Vet J 190 : 34-38 / Vet J 190 : 34 ~ 38
Camma C. / 2012 / Development and validation of fast Real-Time PCR assays for species identification in raw and cooked meat mixtures / Food Control 23 : 400 ~ 404
Crockett AO. / 2001 / Fluorescein-labeled oligonucleotides for real-time PCR: using the inherent quenching of deoxyguanosine nucleotides / Anal Biochem 290 : 89 ~ 97
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