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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 353 - 362 (10page)
이용수
초록· 키워드
오류제보하기
A total of 1,354 samples was collected from bovine and porcine carcass from January 2005 to December 2006 in a slaughter house. Twenty five strains(1.8%) of L monocytogenes were isolated from 1,354 samples using selective media. Ten(1.4%) L monocytogenes were isolated from the 677 of bovine carcasses, and 15(2.2%) were isolated from the 677 of porcine carcasses. Among 15 L monocytogenes from porcine, 11 siolates were serovars 1/2c, followed by 1/2b (3 strains, 20.0%) and 1/2a(1 strain) Out of 10 bovine samples, positive cases in 1/2a were 9 strains (90.0%), 1/2b were 1 strains(10.0%). PCR primers were selected to amplify a 520-base pair(bp) DNA fragment from the listeolysin O gene (hlyA) of L monocytogenes. A 520-bp product was detected in PCR with DNA from L monocytogenes, but not from the other Listeria species tested. *Corresponding author:Phone: +82-2-570-3437, Fax: +82-2-570-3043 E-mail : heedoogy@hanmail.netA total of 25 L monocytogenes strains were analysed by PFGE after digestion with ApaⅠ. PFGE analysis of genomic DNA showed the 14~18 fragments ranging in size from 30 to 550 kb, resulting in 14 patterns.
목차
등록된 정보가 없습니다.
참고문헌 (28)
참고문헌 신청
Bansal NS / 1996 / Development of polymerase chain reaction assay for the detection of Listeria monocyto?genes in foods 22 : 353 ~ 356
Bessesen MT / / Rotbart HA. 1990. Detection of Listeria mono cytogenes by using the polymerase chain reaction. Appl Envir Microbiol 56:2930-2932.
Beumer RR / 1996 / Confirmation and identification of Listeria spp 22 : 448 ~ 452
Broome CV / / Bibb WF. 1991. The epidemiology of listerioses in the United States-1986. Am J Epidemiol 133:392-401.
Bubert A / 1999 / Detection and differentiation of Listeria spp. by a single reaction based on multiplex PCR 65 : 4688 ~ 4692
Bessesen MT / / Rotbart HA. 1990. Detection of Listeria mono cytogenes by using the polymerase chain reaction. Appl Envir Microbiol 56:2930-2932.
Beumer RR / 1996 / Confirmation and identification of Listeria spp 22 : 448 ~ 452
Broome CV / / Bibb WF. 1991. The epidemiology of listerioses in the United States-1986. Am J Epidemiol 133:392-401.
Bubert A / 1999 / Detection and differentiation of Listeria spp. by a single reaction based on multiplex PCR 65 : 4688 ~ 4692
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