지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 51 - 58 (8page)
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Purpose: Tuberculous pleurisy is the most frequent extrapulmonary manifestation of tuberculosis. In spite of adequate treatment, pleural fibrosis is a common complication,but the mechanism has not been elucidated. This study is to determine whether epithelial to mesenchymal transition (EMT) of mesothelial cells occurs in tuberculous pleurisy. Materials and Methods: Normal pleural mesothelial cells, isolated from irrigation fluids during operations for primary spontaneous pneumothorax,were characterized by immunofluorescence and reverse transcription polymerase chain reaction (RT-PCR). These cells were treated in vitro with various cytokines,which were produced in the effluents of tuberculous pleurisy. The isolated cells from the effluents of tuberculous pleurisy were analyzed by immunofluorescence and RT-PCR analysis. Results: The isolated cells from the irrigation fluid of primary spontaneous pneumothorax had epithelial characteristics. These cells, with transforming growth factor-β1 and/or interleukin-1β treatment, underwent phenotypic transition from epithelial to mesenchymal cells, with the loss of epithelial morphology and reduction in cytokeratin and E-cadherin expression. Effluent analysis from tuberculous pleurisy using immunofluorescence and RT-PCR demonstrated two phenotypes that showed mesenchymal characteristics and both epithelial & mesencymal characteristics. Conclusion: Our results suggest that pleural mesothelial cells in tuberculous pleurisy have been implicated in pleural fibrosis through EMT.
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참고문헌 (24)
참고문헌 신청
Aguilera A / 2005 / Epithelial to mesenchymal transition as a triggering factor of peritoneal membrane fibrosis and angiogenesis in peritoneal dialysis patients / Curr Opin
Bariety J / 2003 / Glomerular epithelial-mesenchymal transdifferentiation in pauci-immune crescentic glomerulonephritis / Nephrol Dial Transplant 18 : 1777 ~ 1784
Ceyhan BB / 2003 / Transforming growth factor beta-1 level in pleural effusion / Respirology 8 : 321 ~ 325
Fan JM / 2001
Hay ED / 1995 / An overview of epithelio-mesenchymal transformation / Acta Anat (Basel) 154 : 8 ~ 20
Bariety J / 2003 / Glomerular epithelial-mesenchymal transdifferentiation in pauci-immune crescentic glomerulonephritis / Nephrol Dial Transplant 18 : 1777 ~ 1784
Ceyhan BB / 2003 / Transforming growth factor beta-1 level in pleural effusion / Respirology 8 : 321 ~ 325
Fan JM / 2001
Hay ED / 1995 / An overview of epithelio-mesenchymal transformation / Acta Anat (Basel) 154 : 8 ~ 20
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