지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 1,333 - 1,340 (8page)
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Purpose: The aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase (HDAC) activity in ejaculated sperm and their relevance to conventional sperm parameters. Materials and Methods:Thirty-three normozoospermic men were included in this study. Semen samples were processed by swim-up and then immunostained by six acetylation antibodies (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac). Our preliminary study verified the expression of HAT/HDAC1 in mature human sperm. From vitrified-warmed sperm samples, total HAT/HDAC activity was measured by commerciallyavailable kits. Nuclear DNA integrity was also measured by TUNEL assay. Results: The levels of six acetylation marks were not related with conventional sperm parameters including sperm DNA fragmentation index (DFI) as well as HAT/HDAC activity. However, sperm DFI was positively correlated with HAT activity (r=0.038 after adjustment, p<0.02). HAT activity showed a negative relationshipwith HDAC activity (r=-0.51, p<0.01). Strict morphology was negatively correlated with acetylation enzyme index (=HAT activity/HDAC activity) (r= -0.53, p<0.01). Conclusion: Our works demonstrated a significant relationship of acetylation-associated enzyme activity and strict morphology or sperm DFI.
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참고문헌 (26)
참고문헌 신청
Akiyama T / 2006 / Inadequate histone deacetylation during oocyte meiosis causes aneuploidy and embryo death in mice / Proc Natl Acad Sci U S A 103 : 7339 ~ 7344
Dada R / 2012 / Epigenetics and its role in male infertility / J Assist Reprod Genet 29 : 213 ~ 223
Davie JR / 1998 / Covalent modifications of histones: expression from chromatin templates / Curr Opin Genet Dev 8 : 173 ~ 178
Emery BR / 2006 / The effect of epigenetic sperm abnormalities on early embryogenesis / Asian J Androl 8 : 131 ~ 142
Faure AK / 2003 / Misregulation of histone acetylation in Sertoli cell-only syndrome and testicular cancer / Mol Hum Reprod 9 : 757 ~ 763
Dada R / 2012 / Epigenetics and its role in male infertility / J Assist Reprod Genet 29 : 213 ~ 223
Davie JR / 1998 / Covalent modifications of histones: expression from chromatin templates / Curr Opin Genet Dev 8 : 173 ~ 178
Emery BR / 2006 / The effect of epigenetic sperm abnormalities on early embryogenesis / Asian J Androl 8 : 131 ~ 142
Faure AK / 2003 / Misregulation of histone acetylation in Sertoli cell-only syndrome and testicular cancer / Mol Hum Reprod 9 : 757 ~ 763
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