지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 420 - 428 (9page)
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Purpose: Dexmedetomidine, a full agonist of α_2B-adrenoceptors, is used for analgesia and sedation in the intensive care units. Dexmedetomidine produces an initial transient hypertension due to the activation of post-junctional α_2B-adrenoceptors on vascular smooth muscle cells (SMCs). The aims of this in vitro study were to identify mitogen-activated protein kinase (MAPK) isoforms that are primarily involved in full, α_2B-adrenoceptor agonist, dexmedetomidine-induced contraction of isolated rat aortic SMCs. Materials and Methods: Rat thoracic aortic rings without endothelium were isolated and suspended for isometric tension recording. Cumulative dexmedetomidine (10-9 to 10-6 M) dose-response curves were generated in the presence or absence of extracellular signal-regulated kinase (ERK) inhibitor PD 98059, p38 MAPK inhibitor SB 203580, c-Jun NH_2-terminal kinase (JNK) inhibitor SP 600125, L-type calcium channel blocker (verapamil and nifedipine), and α_2-adrenoceptor inhibitor atipamezole. Dexmedetomidine-induced phosphorylation of ERK, JNK, and p38 MAPK in rat aortic SMCs was detected using Western blotting. Results: SP 600125 (10^-6 to 10^-5 M) attenuated dexmedetomidine-evoked contraction in a concentration-dependent manner, whereas PD 98059 had no effect on dexmedetomidine-induced contraction. SB 203580 (10^-5 M) attenuated dexmedetomidine-induced contraction. Dexmedetomidine-evoked contractions were both abolished by atipamezole and attenuated by verapamil and nifedipine. Dexmedetomidine induced phosphorylation of JNK and p38 MAPK in rat aortic SMCs, but did not induce phosphorylation of ERK. Conclusion: Dexmedetomidine-induced contraction involves a JNK- and p38 MAPK-mediated pathway downstream of α_2-adrenoceptor stimulation in rat aortic SMCs. In addition, dexmedetomidine-induced contractions are primarily dependent on calcium influx via L-type calcium channels.
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참고문헌 (23)
참고문헌 신청
[학술지(정기
Abdel-Latif AA / 2001 / Cross talk between cyclic nucleotides and polyphosphoinositide hydrolysis, protein kinases, and contraction in smooth muscle / Exp Biol Med (Maywood)
Akata T / 2007 / General anesthetics and vascular smooth muscle: direct actions of general anesthetics on cellular mechanisms regulating vascular tone / Anesthesiology 106 :
Bloor BC / 1992 / Effects of intravenous dexmedetomidine in humans. II. Hemodynamic changes / Anesthesiology 77 : 1134 ~ 1142
Carter RW / 2002 / Tyrosine kinases regulate intracellular calcium during alpha (2)-adrenergic contraction in rat aorta / Am J Physiol Heart Circ Physiol 283 : H1673 ~ H1680
Abdel-Latif AA / 2001 / Cross talk between cyclic nucleotides and polyphosphoinositide hydrolysis, protein kinases, and contraction in smooth muscle / Exp Biol Med (Maywood)
Akata T / 2007 / General anesthetics and vascular smooth muscle: direct actions of general anesthetics on cellular mechanisms regulating vascular tone / Anesthesiology 106 :
Bloor BC / 1992 / Effects of intravenous dexmedetomidine in humans. II. Hemodynamic changes / Anesthesiology 77 : 1134 ~ 1142
Carter RW / 2002 / Tyrosine kinases regulate intracellular calcium during alpha (2)-adrenergic contraction in rat aorta / Am J Physiol Heart Circ Physiol 283 : H1673 ~ H1680
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