지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 401 - 412 (12page)
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초록· 키워드
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Purpose: Mesenchymal stem cells (MSCs) are multipotent and give rise to distinctly differentiated cells from all three germ layers. Neuronal differentiation of MSC has great potential for cellular therapy. We examined whether the cluster of mechanically made, not neurosphere, could be differentiated into neuron-like cells by growth factors, such as epidermal growth factor (EGF), hepatocyte growth factor (HGF), and vascular endothelial growth factor (VEGF). Materials and Methods: BMSCs grown confluent were mechanically separated with cell scrapers and masses of separated cells were cultured to form cluster BMSCs. As described here cluster of BMSCs were differentiated into neuron-like cells by EGF, HGF, and VEGF. Differentiated cells were analyzed by means of phase-contrast inverted microscopy, reverse transcriptase-polymerase chain reaction (RT-PCR), immunofluorescence, and immunocytochemistry to identify the expression of neural specific markers. Results: For the group with growth factors, the shapes of neuron-like cells was observable a week later, and two weeks later, most cells were similar in shape to neuron-like cells. Particularly,in the group with chemical addition, various shapes of filament structures were seen among the cells. These culture conditions induced MSCs to exhibit a neural cell phenotype, expressing several neuro-glial specific markers. Conclusion: bone marrow-derived mesenchymal stem cells (BMSCs) could be easily induced to form clusters using mechanical scraping, not neurospheres, which in turn could differentiate further into neuron-like cells and might open an attractive possibility for clinical cell therapy for neurodegenerative diseases. In the future, we consider that neuron-like cells differentiated from clusters of BMSCs are needed to be compared and analyzed on a physiological and molecular biological level with preexisting neuronal cells, and studies on the possibility of their transplantation and differentiation capability in animal models are further required.
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참고문헌 (25)
참고문헌 신청
Azizi SA / 1998 / Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats--similarities to astrocyte grafts / Proc Natl Acad Sci
Brazelton TR / 2000 / From marrow to brain: expression of neuronal phenotypes in adult mice / Science 290 : 1775 ~ 1779
Campagnoli C / 2001 / Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow / Blood 98 : 2396 ~ 2402
Eglitis MA / 1997 / Hematopoietic cells differentiate into both microglia and macroglia in the brains of adult mice / Proc Natl Acad Sci U S A : 4080 ~ 4085
In ‘t Anker PS / 2004 / Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta / Stem Cells 22 : 1338 ~ 1345
Brazelton TR / 2000 / From marrow to brain: expression of neuronal phenotypes in adult mice / Science 290 : 1775 ~ 1779
Campagnoli C / 2001 / Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow / Blood 98 : 2396 ~ 2402
Eglitis MA / 1997 / Hematopoietic cells differentiate into both microglia and macroglia in the brains of adult mice / Proc Natl Acad Sci U S A : 4080 ~ 4085
In ‘t Anker PS / 2004 / Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta / Stem Cells 22 : 1338 ~ 1345
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