지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 92 - 100 (9page)
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Purpose: Cancer stem cells have recently been thought to be closely related to tumor development and reoccurrence. It may be a promising way to cure malignant glioma by using glioma stem cell-targeted dendritic cells as a tumor vaccine. In this study, we explored whether pulsing dendritic cells with antigens of glioma stem cells was a potent way to induce specific cytotoxic T lymphocytes and anti-tumor immunity. Materials and Methods: Cancer stem cells were cultured from glioma cell line U251. Lysate of glioma stem cells was obtained by the repeated freezing and thawing method. Dendritic cells (DCs) were induced and cultured from the murine bone marrow cells, the biological characteristics were detected by electron microscope and flow cytometry. The DC vaccine was obtained by mixing DCs with lysate of glioma stem cells. The DC vaccine was charactirizated through the mixed lymphocyte responses and cell killing experiment in vitro. Level of interferon-γ (IFN-γ) in the supernatant was checked by ELISA. Results: After stimulation of lysate of glioma stem cell, expression of surface molecules of DC was up-regulated, including CD80, CD86, CD11C and MHC-II. DCs pulsed with lysate of glioma stem cells were more effective than the control group in stimulating original glioma cells-specific cytotoxic T lymphocytes responses, killing glioma cells and boosting the secretion of IFN-γ in vitro. Conclusion: The results demonstrated DCs loaded with antigens derived from glioma stem cells can effectively stimulate naive T cells to form specific cytotoxic T cells, kill glioma cells cultured in vitro.
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참고문헌 (26)
참고문헌 신청
Ashley DM / 1997 / Bone marrow-generated dendritic cells pulsed with tumor extracts or tumor RNA induce antitumor immunity against central nervous system tumors / J Exp Med
Bernard DJ / 1992 / Effect of epidermal growth factor in HLA class I and class II transcription and protein expression in human breast adenocarcinoma cell lines / Br J Cance
Dey M / 2011 / Cancer stem cells: the final frontier for glioma virotherapy / Stem Cell Rev 7 : 119 ~ 129
Fecci PE / 2003 / The history, evolution, and clinical use of dendritic cell-based immunization strategies in the therapy of brain tumors / J Neurooncol 64 : 161 ~ 176
Gao JX / 2008 / Cancer stem cells: the lessons from pre-cancerous stem cells / J Cell Mol Med 12 : 67 ~ 96
Bernard DJ / 1992 / Effect of epidermal growth factor in HLA class I and class II transcription and protein expression in human breast adenocarcinoma cell lines / Br J Cance
Dey M / 2011 / Cancer stem cells: the final frontier for glioma virotherapy / Stem Cell Rev 7 : 119 ~ 129
Fecci PE / 2003 / The history, evolution, and clinical use of dendritic cell-based immunization strategies in the therapy of brain tumors / J Neurooncol 64 : 161 ~ 176
Gao JX / 2008 / Cancer stem cells: the lessons from pre-cancerous stem cells / J Cell Mol Med 12 : 67 ~ 96
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