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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 486 - 495 (10page)
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초록· 키워드
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Purpose: To investigate the effect of ultra low molecular weight heparin (ULMWH) on glutamate induced apoptosis in rat cortical cells and to explore the possible mechanisms. Materials and Methods: Cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Apoptosis was first analyzed with Hoechst 33258 and then confirmed by DNA fragmentation. The concentration of free intracellular calcium ([Ca²+]i) was determined with fura-2/AM fluorometry. The expression of Bcl-2 family protein and caspase-3 were evaluated with Western blot. Results: Typical apoptotic morphological change in rat cortical cells treated with 100μmol/L glutamate for 24h was detected by Hoechst 33258 staining, which was then confirmed by the DNA ladder of agarose gel electrophoresis. The apoptotic rate of the glutamate treated cells was up to 33.21%, and 24 h of treatment with glutamate increased [Ca²+]i, down-regulated Bcl-2 expression, up-regulated Bax expression, and increased caspase-3 activation in rat cortical cells. Our research demonstrated that ULMWH pretreatment can prevent the glutamate- induced apoptosis, attenuate the increase of [Ca²+]i not only in medium containing Ca²+ but also in Ca²+-free medium, up-regulate the expression of Bcl-2, down-regulate the expression of Bax, and decrease caspase-3 activation. Conclusion: ULMWH has neuroprotective capacity to antagonize glutamate-induced apoptosis in cortical cells, through decrease of Ca²+ release and modulation of apoptotic processes.
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참고문헌 (26)
참고문헌 신청
Ankarcrona M / 1995 / Glutamate-induced neuronal death: a succession of necrosis or apoptosis depending on mitochondrial function / Neuron 15 : 961 ~ 973
Bergamaschini L / 2004 / Peripheral treatment with enoxaparin, a low molecular weight heparin, reduces plaques and beta-amyloid accumulation in a mouse model of Alzheimer's d
Bleich S / 2003 / Glutamate and the glutamate receptor system: a target for drug action / Int J Geriatr Psychiatry 18 (1) : s33 ~ s40
Cavaliere F / 2001 / Hypoglycaemia-induced cell death: features of neuroprotection by the P2 receptor antagonist basilen blue / Neurochem Int 38 : 199 ~ 207
Choi DW / 1987 / Ionic dependence of glutamate neurotoxicity / Neurosci 7 : 369 ~ 379
Bergamaschini L / 2004 / Peripheral treatment with enoxaparin, a low molecular weight heparin, reduces plaques and beta-amyloid accumulation in a mouse model of Alzheimer's d
Bleich S / 2003 / Glutamate and the glutamate receptor system: a target for drug action / Int J Geriatr Psychiatry 18 (1) : s33 ~ s40
Cavaliere F / 2001 / Hypoglycaemia-induced cell death: features of neuroprotection by the P2 receptor antagonist basilen blue / Neurochem Int 38 : 199 ~ 207
Choi DW / 1987 / Ionic dependence of glutamate neurotoxicity / Neurosci 7 : 369 ~ 379
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