지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 449 - 458 (10page)
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Purpose: Candida albicans is an opportunistic pathogen that is commonly found in human microflora. Biofilm formation (BF) is known as a major virulence factor of C. albicans. The aim of this study was to examine the influence of bacterial presence on biofilm formation of C. albicans. Materials and Methods: The BF of Candida was investigated when it was co-cultured with C. albicans (C. albicans53, a yeast with a low BF ability, and C. albicans 163, a yeast with high BF ability) and bacteria. BF was assessed with XTT reduction assay. A scanning electronmicroscope was used to determine the structure of the biofilm, and real-time reverse transcriptase polymerase chain reaction was used to amplify and quantify hyphae-associated genes. Results: Co-culturing with two different types of bacteriaincreased the BF value. Co-culturing with C. albicans 53 and 163 also increasedthe BF value compared to the value that was obtained when the C. albicanswas cultured individually. However, co-culturing with bacteria decreased the BF value of C. albicans, and the BF of C. albicans 163 was markedly inhibited. The expression of adherence and morphology transition related genes were significantlyinhibited by co-culturing with live bacteria. Conclusion: Bacteria have a negative effect on the formation of biofilm by C. albicans. This mechanism is the result of the suppression of genes associated with the hyphae transition of C. albicans,and bacteria particles physically affected the biofilm architecture and biofilm formation.
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참고문헌 (28)
참고문헌 신청
Aas JA / 2005 / Defining the normal bacterial flora of the oral cavity / J Clin Microbiol 43 : 5721 ~ 5732
Baillie GS / 1999 / Role of dimorphism in the development of Candida albicans biofilms / J Med Microbiol 48 : 671 ~ 679
Bandara HM / 2009 / Escherichia coli and its lipopolysaccharide modulate in vitro Candida biofilm formation / J Med Microbiol 58 (Pt 12) : 1623 ~ 1631
Birse CE / 1993 / Cloning and characterization of ECE1, a gene expressed in association with cell elongation of the dimorphic pathogen Candida albicans / Infect Immun 61 : 3
Chandra J / 2001 / Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance / J Bacteriol 183 : 5385 ~ 5394
Baillie GS / 1999 / Role of dimorphism in the development of Candida albicans biofilms / J Med Microbiol 48 : 671 ~ 679
Bandara HM / 2009 / Escherichia coli and its lipopolysaccharide modulate in vitro Candida biofilm formation / J Med Microbiol 58 (Pt 12) : 1623 ~ 1631
Birse CE / 1993 / Cloning and characterization of ECE1, a gene expressed in association with cell elongation of the dimorphic pathogen Candida albicans / Infect Immun 61 : 3
Chandra J / 2001 / Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance / J Bacteriol 183 : 5385 ~ 5394
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