지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 610 - 615 (6page)
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초록· 키워드
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Purpose: Deposition of polymeric IgA1 in the kidney mesangium is the hallmark of IgA nephropathy, but the molecular mechanisms of IgA-mediated mesangial responses and inflammatory injuries remain poorly understood. We hypothesize that Toll-like receptor 4 (TLR4) is involved in IgA-induced mesangial cell activation. Materials and Methods: Mouse mesangial cells were stimulated with lipopolysaccharide (LPS) (1 μg/mL), IgA (20 μg/mL), or both, and TLR4 expression was measured by real time RT-PCR and Western blot. Intracellular responses to LPS or IgA were assessed by Western blot for ERK1/2, JNK, p38 MAP kinases (MAPKs), Iκ-Bα degradation and fibronectin secretion. MCP-1 secretion was assessed by ELISA. Small interfering RNA (siRNA) of TLR4 was used to confirm that the effects were caused by TLR4 activity. Results: LPS- or IgA-treatment upregulated the levels of TLR4 mRNA and protein in cultured MMC at 24 h. LPS and IgA induced rapid phosphorylation of MAPKs, but degradation of Iκ-Bα was observed only in LPS-treated MMC. LPS, but not IgA, induced increased secretion of MCP-1 and fibronectin at 24 h or 48 h. Combined LPS and IgA treatment did not cause additional increases in TLR4 mRNA and protein levels or Iκ-Bα degradation,and MCP-1 and fibronectin secretions were less than with LPS alone. LPS- or IgA-induced TLR4 protein levels and MAPK activation were inhibited by transfection with TLR4 siRNA. Conclusion: These results indicate that the activation of MAPKs and MCP-1 secretion are mediated by TLR4, at least in part, in IgA-treated mesangial cells. TLR4 is involved in mesangial cell injury by induction of pro-inflammatory cytokines in IgA nephropathy.
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참고문헌 (21)
참고문헌 신청
Akira S / 2006 / Pathogen recognition and innate immunity / Cell 124 : 783 ~ 801
Allen AC / 1995 / Galactosylation of N- and O-linked carbohydrate moieties of IgA1 and IgG in IgA nephropathy / Clin Exp Immunol 100 : 470 ~ 474
Anders HJ / 2007 / Toll-like receptors: emerging concepts in kidney disease / Curr Opin Nephrol Hypertens 16 : 177 ~ 183
Arima S / 1991 / Significance of mononuclear phagocytes in IgA nephropathy / Kidney Int 39 : 684 ~ 692
Brown HJ / 2007 / Toll-like receptor 4 ligation on intrinsic renal cells contributes to the induction of antibody-mediated glomerulonephritis via CXCL1 and CXCL2 / J Am Soc N
Allen AC / 1995 / Galactosylation of N- and O-linked carbohydrate moieties of IgA1 and IgG in IgA nephropathy / Clin Exp Immunol 100 : 470 ~ 474
Anders HJ / 2007 / Toll-like receptors: emerging concepts in kidney disease / Curr Opin Nephrol Hypertens 16 : 177 ~ 183
Arima S / 1991 / Significance of mononuclear phagocytes in IgA nephropathy / Kidney Int 39 : 684 ~ 692
Brown HJ / 2007 / Toll-like receptor 4 ligation on intrinsic renal cells contributes to the induction of antibody-mediated glomerulonephritis via CXCL1 and CXCL2 / J Am Soc N
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