지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 43 - 49 (7page)
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초록· 키워드
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Objective: Pyungwi-san (PWS) plays a role in a number of physiologic and pharmacologic functions in many organs. Interstitial cells of Cajal (ICCs) are pacemaker cells that generate slow waves in the gastrointestinal (GI) tract. We aimed to investigate the beneficial effects of PWS in mouse small-intestinal ICCs.
Methods: Enzymatic digestion was used to dissociate ICCs from the small intestine of a mouse. The wholecell patch-clamp configuration was used to record membrane potentials from the cultured ICCs.
Results: ICCs generated pacemaker potentials in the GI tract. PWS produced membrane depolarization in the current clamp mode. Pretreatment with a Ca2+-free solution and a thapsigargin, a Ca2+-ATPase, inhibitor in the endoplasmic reticulum, eliminated the generation of pacemaker potentials. However, only when the thapsigargin was applied in a bath solution, the membrane depolarization was not produced by PWS.
Furthermore, the membrane depolarizations due to PWS were inhibited not by U-73122, an active phospholipase C inhibitor, but by chelerythrine and calphostin C, protein kinase C inhibitors.
Conclusions: These results suggest that PWS might affect GI motility by modulating the pacemaker activity in the ICCs.
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참고문헌 (20)
참고문헌 신청
Aiello EA / 1996 / Protein kinase C inhibits delayed rectifier K+ current in rabbit vascular smooth muscle cells / Am J Physiol 271 (1 Pt 2) : H109 ~ H119
Goto K / 2004 / Two types of spontaneous depolarizations in the interstitial cells freshly prepared from the murine small intestine / J Physiol 559 (Pt 2) : 411 ~ 422
Heo J / 1994 / Dongeuibogam / Namsandang : 435 ~
Huizinga JD / 1995 / W/kit gene required for interstitial cells of Cajal and for intestinal pacemaker activity / Nature 373 (6512) : 347 ~ 349
Huizinga JD / 2002 / High-conductance chloride channels generate pacemaker currents in interstitial cells of Cajal / Gastroenterology 123 (5) : 1627 ~ 1636
Goto K / 2004 / Two types of spontaneous depolarizations in the interstitial cells freshly prepared from the murine small intestine / J Physiol 559 (Pt 2) : 411 ~ 422
Heo J / 1994 / Dongeuibogam / Namsandang : 435 ~
Huizinga JD / 1995 / W/kit gene required for interstitial cells of Cajal and for intestinal pacemaker activity / Nature 373 (6512) : 347 ~ 349
Huizinga JD / 2002 / High-conductance chloride channels generate pacemaker currents in interstitial cells of Cajal / Gastroenterology 123 (5) : 1627 ~ 1636
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