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자료유형
학술저널
저자정보
저널정보
대한약침학회 Journal of Pharmacopuncture Journal of Pharmacopuncture 제6권 제2호
발행연도
2003.1
수록면
45 - 56 (12page)

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Objectives:The purpose of this study was to investigate the effects of Bee Venom on the lipopolysaccharide(LPS), sodium nitroprusside(SNP), hydrogen peroxide(H2O2)-induced expression inducilble nitric oxide synthetase(iNOS), tumor necrosis factor-α(TNF-α) and nuclear factor kappa B(NF-kB) in RAW 264.7 cells, a murine macrophage cell line. Methods:The expressions of expression iNOS and TNF-α were determined by western blotting with corresponding antibodies. The expressions of expression NF-kB was assayed by EMSA method. Results:1. The 0.5, 1 and 5 ㎍/㎖ of bee venom on LPS-induced expression of iNOS, the 5 ㎍/㎖ of bee venom on SNP-induced expression of iNOS and the 1 ㎍/㎖ of bee venom on H2O2-induced expression of iNOS compared with control were inhibited significantly. 2. The 0.5, 1 and 5 ㎍/㎖ of bee venom inhibited significantly LPS and H2O2-induced expression of TNF-α compared with control, respectively. The 0.5 ㎍/㎖ of bee venom increased significantly SNP-induced expression of TNF-α compared with control. 3. The 5 ㎍/㎖ of bee venom on LPS-induced expression of NF-kB, the 0.5 ㎍/㎖ of bee venom on SNP-induced expression of NF-kB and the 0.5, 5 ㎍/㎖ of bee venom on H2O2-induced expression of NF-kB were inhibited significantly compared with control, respectively.

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