지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 130 - 136 (7page)
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초록· 키워드
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Purpose: Multiplex PCR can allow multiple pathogens to be detected in the same reaction tube, saving time and reagents. The aim of this study was to evaluate the multiplex PCR kit (Seegene, Korea) for detection of fastidious microorganisms such as six sexually transmitted pathogens.
Materials and Methods: Using clinical specimens, multiplex PCR and single PCR were used to test for Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Neisseria gonorrhoeae,Trichomonas vaginalis and Ureaplasma urealyticum.
Results: Multiplex PCR had an overall sensitivity of 97.2% and specificity of 100% compared to the single PCR. The limits of detection for the multiplex PCR and single PCR were from 3.03×10-6 to 7.06×10-7 μg/mL and from 3.03×10-6 to 3.03×10-31 μg/mL, respectively.
Conclusions: The multiplex PCR kit has considerable potential to use as a routine method in clinical laboratories. Before introducing the new multiplex PCR method in the laboratory, thorough evaluation and validation of multiplex PCR is essential. To achieve reliable results from multiplex PCR, feasible guidelines,standardization and quality control are of major importance.
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참고문헌 (17)
참고문헌 신청
Chamberlain JS / 1988 / Deletion screening of the duchenne muscular dystrophy locus via multiplex DNA amplification / Nucleic Acids Res 16 (23) : 11141 ~ 11156
Chun JY / 2007 / Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and SNP genotyping of CYP2C19 gene / Nucleic Acids Res 35 (6) : e40 ~
Elnifro EM / 2000 / Multiplex PCR: optimization and application in diagnostic virology / Clin Microbiol Rev 13 (4) : 559 ~ 570
Higgins RR / 2009 / Verification of the ProPneumo-1 assay for the simultaneous detection of mycoplasma pneumoniae and chlamydophila pneumoniae in clinical respiratory specim
Higgins RR / 2011 / Evaluation and verification of the seeplex diarrhea-V ACE assay for simultaneous detection of adenovirus, rotavirus, and norovirus genogroups I and II in
Chun JY / 2007 / Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and SNP genotyping of CYP2C19 gene / Nucleic Acids Res 35 (6) : e40 ~
Elnifro EM / 2000 / Multiplex PCR: optimization and application in diagnostic virology / Clin Microbiol Rev 13 (4) : 559 ~ 570
Higgins RR / 2009 / Verification of the ProPneumo-1 assay for the simultaneous detection of mycoplasma pneumoniae and chlamydophila pneumoniae in clinical respiratory specim
Higgins RR / 2011 / Evaluation and verification of the seeplex diarrhea-V ACE assay for simultaneous detection of adenovirus, rotavirus, and norovirus genogroups I and II in
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